Proteomic analysis of rat liver proteins differentially induced by trichloroethylene
Proteomic analysis of rat liver proteins differentially induced by trichloroethylene
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DOI：
10.1007/s13206-010-4109-9
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发表时间：
2010-03-20
期刊：
影响因子：
4.3
通讯作者：
Lee, Mi-Young
中科院分区：
文献类型：
--
作者：
Park, Seul-Ki;Nam, Suk-Woo;Lee, Mi-Young
三氯乙烯（TCE）是一种挥发性和亲脂性溶剂，广泛应用于工业溶剂中，被认为对人体有毒。为了确定早期TCE诱导的肝毒性的可能的蛋白质生物标志物，我们用相当于LD 50的10%的单剂量TCE治疗大鼠，并在给药后72小时分析其肝脏蛋白质组。我们鉴定了8种肝脏蛋白，其表现出TCE诱导的表达水平变化至少2倍;其中3种蛋白上调，其余蛋白下调。由于这些蛋白质在体内没有明显肝毒性的情况下大量发生显著变化，因此它们可能是TCE暴露的早期预测生物标志物的候选者之一。
Trichloroethylene (TCE), a volatile and lipophilic solvent widely used in industrial degreasing applications, is believed to be toxic in humans. To identify possible protein biomarkers of early TCE-induced hepatotoxicity, we treated rats with a single dose of TCE equivalent to 10% of LD50 and analyzed their liver proteomes 72 h after dosing. We identified eight liver proteins that exhibited a TCE-induced change in expression level of at least 2-fold; three of these proteins were upregulated and the rest were downregulated. Because these proteins changed significantly in abundance in the absence of notable hepatotoxicity in vivo, they may be useful as one of the candidates of early predictive biomarkers of TCE exposure in rats.