Microwave Sensing of Yeast Cell Species and Viability
Microwave Sensing of Yeast Cell Species and Viability
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DOI：
10.1109/tmtt.2020.3048176
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发表时间：
2021-03
影响因子：
4.3
通讯作者：
Jeffrey A. Osterberg;Neelima Dahal;R. Divan;C. S. Miller;David Moline;T. Caldwell;Xianzhong Yu;S. Harcum;Pingshan Wang
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文献类型：
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作者：
Jeffrey A. Osterberg;Neelima Dahal;R. Divan;C. S. Miller;David Moline;T. Caldwell;Xianzhong Yu;S. Harcum;Pingshan Wang
我们报道了一种简单的基于干涉仪的微波传感系统的开发，用于流动酵母细胞的多频率表征和分化。该干涉仪使用一条简单的微带线，与微流控通道集成在一起，用于单细胞测量。利用高频结构模拟器(HFSS)从测量的散射参数中提取复介电常数(F)=varepsilon‘(F)-j varepsilon’‘(F)，并用高频结构模拟器(HFSS)进行了验证。通过测量不同直径和不同干涉仪工作频率下的聚苯乙烯颗粒，对传感系统和算法进行了评价。在这些频率下，对活的和不活的酿酒酵母和巴氏酵母的细胞进行了测定。结果表明，每种酵母的频率相关介电常数和活性以及不同类型酵母之间的频率相关介电常数差异。在某些频率点上的差异是显著的，并使混合悬液中的细胞能够分化，这一点也通过本文建立的预测模型进行了验证。
We report the development of a simple interferometer-based microwave sensing system for multiple frequency characterization and differentiation of in-flow yeast cells. The interferometer uses a simple microstrip line, integrated with a microfluidic channel, for single-cell measurement. An algorithm was developed and verified with high-frequency structure simulator (HFSS) for complex permittivity, $\varepsilon ^{\ast }(f) = \varepsilon '(f)- j\varepsilon ''(f)$ , extraction from measured scattering parameters. The sensing system and the algorithm were evaluated by measuring polystyrene particles of different diameters and at different interferometer operating frequencies. Viable and nonviable Saccharomyces cerevisiae and Saccharomyces pastorianus cells were measured at those frequencies. The results showed frequency-dependent permittivity values for each species of yeast and viability as well as frequency-dependent permittivity differences between different yeast types. The differences at some frequency points are significant and enable the differentiation of cells in mixed suspension, which is also demonstrated with a prediction model developed in this work.