Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds
Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds
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DOI：
10.3390/colloids2020015
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发表时间：
2018-06-01
影响因子：
2.4
通讯作者：
Ito, Norihiko
中科院分区：
文献类型：
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作者：
Izawa, Hironori;Okuda, Noriko;Ito, Norihiko
可以容易地制造的微观表面结构已经被要求作为组织工程的机械结构线索。预期通过研磨合成的微观表面浮雕作为细胞培养支架用于细胞增殖、控制细胞排列和分化以及球体生成。我们以前开发的生物基褶皱薄膜制备通过木质化模拟反应和干燥。虽然这些膜预期作为细胞培养支架的候选物，但未探索在水性缓冲溶液中褶皱表面的稳定性和形态。在这里，我们调查的褶皱膜在磷酸盐缓冲液中的表面形态，和他们的应用3T3细胞培养。在40 ° C下用浸渍处理制备的褶皱膜即使在5天后在磷酸盐缓冲盐水中也保持其褶皱结构，尽管褶皱由于皮肤层的水合作用而变宽。有趣的是，在3T3细胞培养物中，使用褶皱膜比使用具有相同表面组成的平坦膜观察到更高的细胞数量。此外，通过体内实验证实了褶皱膜的高生物相容性。这些结果强烈鼓励应用褶皱膜作为机械结构线索。褶皱膜的高级应用研究正在进行中。
Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 degrees C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.