Development of an Enzyme-Linked Immunosorbent Assay to Identify Host-Feeding Preferences of Phlebotomus Species (Diptera: Psychodidae) in Endemic Foci of Visceral Leishmaniasis in Nepal
Development of an Enzyme-Linked Immunosorbent Assay to Identify Host-Feeding Preferences of Phlebotomus Species (Diptera: Psychodidae) in Endemic Foci of Visceral Leishmaniasis in Nepal
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DOI：
10.1603/me09184
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发表时间：
2010-09-01
影响因子：
2.1
通讯作者：
Cameron, Mary
中科院分区：
文献类型：
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作者：
Burniston, Ian;Roy, Lalita;Cameron, Mary
人类内脏利什曼病是由白纹伊蚊(Phlebotomus argentipe Annandale&Brunetti)沙蝇传播的一种严重危害印度次大陆公共卫生的疾病。了解媒介的摄食行为可用于调查干预计划期间人与媒介接触的变化。对酶联免疫吸附试验(EL ISA)进行了改进，使其适用于白纹伊蚊和白纹伊蚊(双翅目：心蝇科)血粉的来源鉴定。比较了沉淀素环试验和酶联免疫吸附试验的敏感性和特异性，以及血粉消化不同阶段试验的稳定性。与沉淀素试验相比，酶联免疫吸附试验对血粉来源的鉴定更为敏感和特异。当使用平板阅读器时，可获得100%的灵敏度和特异度。当比较不同消化阶段的技术时，发现沉淀素和目测酶联免疫吸附试验的敏感度分别在餐后48小时和72小时下降。然而，使用平板阅读器的ELISA法的灵敏度不会因消化时间而改变。用开发的酶联免疫吸附试验测定尼泊尔Terai地区的P.argentipe和P.Papatasi的食性，表明P.Papatasi具有高度的亲人性，而P.argentipe似乎以人和动物，特别是牛为食。
Anthroponotic visceral leishmaniasis, transmitted by Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) sand flies, is regarded as a major problem of public health importance in the Indian subcontinent. Understanding the feeding behavior of the vector can be used to investigate changes in human-vector contact during intervention programs. An enzyme-linked immunosorbent assay (ELISA) was modified to make it suitable to identify the origin of P. argentipes and Phlebotomus papatasi Scopoli (Diptera: Psychodidae) blood meals. The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. When using the ELISA method with a plate reader, it was possible to obtain 100% sensitivity and specificity. When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. However, the sensitivity of the ELISA using a plate reader was not altered by the digestion time. The feeding habits of P. argentipes and P. papatasi from the Terai region of Nepal, determined by the ELISA developed, showed P. papatasi to be highly anthropophilic, and P. argentipes appeared to feed both on humans and animals, in particular bovines.