Genome-wide identification of MYB genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.
Genome-wide identification of MYB genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.
复制标题
向日葵MYB基因的全基因组鉴定及不同生物和非生物胁迫下的表达分析。
DOI：
10.1016/j.indcrop.2019.111924
复制
发表时间：
2020-01-01
影响因子：
5.9
通讯作者：
Xu, Ling
中科院分区：
文献类型：
--
作者：
Li, Juanjuan;Liu, Hui;Xu, Ling
成髓细胞瘤（myeloblastosis，MYB）超家族是转录因子（transcript factors，TF）中最大的家族之一，在植物中具有多种功能，包括调节植物对生物和非生物胁迫的应答。在这项研究中，我们进行了向日葵（向日葵L.）的MYB家族的全基因组特征。共鉴定出245个MYB候选基因，分为10类。HaMYB基因的基因组定位和旁系同源物将245个基因定位在17条染色体上，基因间存在共线性关系。对HaMYBs的保守结构域、基因结构和进化关系进行了分析。向日葵品种TK 0409的幼苗（对列当库马纳感病品种）和JY 207（抗性品种）施加到三个水平（0、10%和20%）使用聚乙二醇的模拟干旱胁迫处理（PEG 6000）、三个水平（0、150和300 mM NaCl）的盐度处理和两个水平（存在和不存在）的列当生物胁迫处理。研究了55个选择的HaMYB基因在duff ;erent胁迫下的表达模式。qRT-PCR分析结果表明，向日葵中许多MYB基因在不同胁迫处理下的表达量存在显著差异，这表明HaMYB基因具有作为提高向日葵抗逆性的靶基因的潜力。有趣的是，在非生物胁迫下，HaMYB 16.1在PEG模拟干旱胁迫下表现出较高的表达水平，但在盐胁迫下，根和叶中的MYB基因HaMYB 15.6，HaMYB 15.13，HaMYB 15.14和HaMYB 58的转录水平随着盐浓度的增加而显著增强，而HaMYB 15.13，叶片中HaMYB52.2和HaMYB52.4的含量也随着PEG浓度的增加而显著提高。在生物胁迫下，HaMYB 3.1和14 aMYB 15.12在根和叶中的表达量均较高。TK 0409在根和叶中的表达量较低。JY207.这些结果表明，MYB基因的积极参与响应生物和非生物胁迫。通过对向日葵MYB基因家族的全基因组鉴定和表达分析，不仅为向日葵逆境响应机制的研究提供了重要信息，也为向日葵的进一步改良提供了候选基因。
The myeloblastosis (MYB) superfamily, which has diverse functions in plants including regulating responses to biotic and abiotic stresses, is one of the largest families of transcript factors (TFs). In this study, we have conducted genome-wide characterization of the MYB family in sunflower (Helianthus annuus L.). A total of 245 genes were identified as candidate MYB genes, which were classified into 10 groups. Genomic localization and paralogs of HaMYB genes mapped the 245 genes on 17 chromosomes with collinearity relationship among genes. Conserved domain, gene structure, and evolutionary relationships of HaMYBs were established and analyzed. Seedlings of sunflower cultivars TK0409 (a sensitive cultivar to Orobanche cumana infection) and JY207 (a resistant cultivar) were imposed to three levels (0, 10%, and 20%) of simulated drought stress treatments using polyethylene glycol (PEG 6000), three levels (0, 150, and 300 mM NaCl) of salinity treatments and two levels (presence and absence) of Orobanche cumona biotic stress treatments. The expression patterns of 55 selected HaMYB genes were investigated for duff ;erent stresses. The results from qRT-PCR analyses revealed that many MYB genes In sunflower had significant differences in expression under the different stress treatments, which highlighted the potential of using HaMYB genes as targets for improving stress tolerance in sunflower. Interestingly, under abiotic stress, HaMYB16.1 exhibited higher expression level under simulated drought stress by PEG but was extremely down-regulated under salt stress both in roots and leaves, the transcript levels of the MYB genes HaMYB15.6, HaMYB15.13, HaMYB15.14, and HaMYB58 were significantly enhanced with the increase of salinity concentration in roots, while the expression of HaMYB15.13, HaMYB52.2 and HaMYB52.4 in leaves were also considerably enhanced with the increase of PEG concentration from 10% to 20%. However, under biotic stress, HaMYB3.1 and 14aMYB15.12 exhibited higher expression in both roots and leaves of cv. TK0409 but low level of expression in both roots and leaves of cv. JY207. These results indicated the active involvement of MYB genes in response to biotic and abiotic stresses. With this better knowledge on genome-wide identification and expression analyses of MYB gene family in sunflower, this study provides not only important information for stress response mechanism in the crop but also candidate genes for the future improvement of this crop.