Creation and functional analysis of new Puroindoline alleles in Triticum aestivum
Creation and functional analysis of new Puroindoline alleles in Triticum aestivum
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DOI：
10.1007/s00122-008-0893-1
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发表时间：
2009-01-01
影响因子：
5.4
通讯作者：
Giroux, M. J.
中科院分区：
文献类型：
--
作者：
Feiz, L.;Martin, J. M.;Giroux, M. J.
小麦(Triticum aestivum L.)的硬度(Ha)基因控制着籽粒质地，影响着小麦的许多最终用途特性。Ha基因座的功能由Puroindoline a和b基因Pina和Pinb组成。Pin等位基因多样性的缺乏是限制Ha功能分析和小麦品质改良的主要因素。为了创造新的Ha等位基因，利用乙基甲烷磺酸盐诱变技术，在软白春品种AlPowa中产生了一个630个成员的M-2群体。对M-2群体进行筛选，以确定Pina和Pinb的新等位基因。共鉴定出18个新的Pin等位基因，其中包括8个错义等位基因。四个新的Pin等位基因的F-2群体是在每个Pin等位基因与非突变的AlPowa杂交后形成的。然后测定了F-2：3种子的籽粒硬度，并量化了每个等位基因对籽粒硬度的影响。被测突变对F-2群体籽粒硬度变异的贡献率在28%到94%之间，所有突变系的粒重和活力都得到了恢复。通过直接表型鉴定或直接测序筛选新的Pin等位基因是发现新的Ha等位基因的有效途径，有助于小麦品质改良和了解Ha座位的功能。
The Hardness ( Ha) locus controls grain texture and affects many end-use properties of wheat ( Triticum aestivum L.). The Ha locus is functionally comprised of the Puroindoline a and b genes, Pina and Pinb, respectively. The lack of Pin allelic diversity is a major factor limiting Ha functional analyses and wheat quality improvement. In order to create new Ha alleles, a 630 member M-2 population was produced in the soft white spring cultivar Alpowa using ethylmethane sulfonate mutagenesis. The M-2 population was screened to identify new alleles of Pina and Pinb. Eighteen new Pin alleles, including eight missense alleles, were identified. F-2 populations for four of the new Pin alleles were developed after crossing each back to non-mutant Alpowa. Grain hardness was then measured on F-2:3 seeds and the impact of each allele on grain hardness was quantified. The tested mutations were responsible for between 28 and 94% of the grain hardness variation and seed weight and vigor of all mutation lines was restored among the F-2 populations. Selection of new Pin alleles following direct phenotyping or direct sequencing is a successful approach to identify new Ha alleles useful in improving wheat product quality and understanding Ha locus function.