Distribution of orexin neurons in the adult rat brain
Distribution of orexin neurons in the adult rat brain
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DOI：
10.1016/s0006-8993(99)01336-0
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发表时间：
1999-05-08
期刊：
影响因子：
2.9
通讯作者：
Goto, K
中科院分区：
文献类型：
--
作者：
Nambu, T;Sakurai, T;Goto, K
食欲素（Orexin，ORX）-A和-B是最近发现的神经肽，它们特异性地定位于下丘脑外侧区（lateral hypothalamic area，LHA）和下丘脑背内侧核（dormedial hypothalamic nucleus，DMH）内和周围的神经元中，这些区域与摄食行为有关。在这里，我们报告的ORX神经元在成年大鼠大脑中的分布的进一步研究，提供一个一般概述的ORX神经元系统。免疫组化研究表明，ORX免疫反应（IR）神经元特异性定位于下丘脑，包括穹窿周围核，LHA，DMH，和下丘脑后区。ORX-IR轴突及其静脉曲张的终端显示了广泛的分布在整个成年大鼠脑。在整个下丘脑，包括弓状核和室旁核，与摄食行为的调节有关的区域，观察到ORX-ir神经末梢。我们还在下丘脑外的区域中观察到ORX-1 R静脉曲张终末的强染色，包括大脑皮质、丘脑内侧组、室周器官（穹窿下器官和最后区）、边缘系统（海马、杏仁核和灰核）和脑干（蓝斑和中缝核）。这些结果表明，ORX系统提供了下丘脑和其他脑区之间的联系，并含有ORX的LHA和DMH神经元在整合复杂的生理学基础的摄食行为中发挥重要作用。(C)1999 Elsevier Science B. V.保留所有权利。
Orexin (ORX)-A and -B are recently identified neuropeptides, which are specifically localized in neurons within and around the lateral hypothalamic area (LHA) and dorsomedial hypothalamic nucleus (DMH), the regions classically implicated in feeding behavior. Here, we report a further study of the distribution of ORX-containing neurons in the adult rat brain to provide a general overview of the ORX neuronal system. Immunohistochemical study using anti-ORX antiserum showed ORX-immunoreactive (ir) neurons specifically localized within the hypothalamus, including the perifornical nucleus, LHA, DMH, and posterior hypothalamic area. ORX-ir axons and their varicose terminals showed a widespread distribution throughout the adult rat brain. ORX-ir nerve terminals were observed throughout the hypothalamus, including the arcuate nucleus and paraventricular hypothalamic nucleus, regions implicated in the regulation of feeding behavior. We also observed strong staining of ORX-ir varicose terminals in areas outside the hypothalamus, including the cerebral cortex, medial groups of the thalamus, circumventricular organs (subfornical organ and area postrema), Limbic system (hippocampus, amygdala, and indusium griseum), and brain stem (locus coeruleus and raphe nuclei). These results indicate that the ORX system provides a link between the hypothalamus and other brain regions, and that ORX-containing LHA and DMH neurons play important roles in integrating the complex physiology underlying feeding behavior. (C) 1999 Elsevier Science B.V. All rights reserved.