Clonally expanded lymphocytes in the minor salivary glands of Sjögren's syndrome patients without lymphoproliferative disease.
Clonally expanded lymphocytes in the minor salivary glands of Sjögren's syndrome patients without lymphoproliferative disease.
复制标题
无淋巴细胞增殖性疾病的干燥综合征患者小唾液腺中克隆性扩增的淋巴细胞。
DOI：
10.1002/art.1780371006
复制
发表时间：
1994
影响因子：
--
通讯作者：
J. Gómez
中科院分区：
文献类型：
--
作者：
J. Pablos;P. Carreira;L. Morillas;G. Montalvo;C. Ballestín;J. Gómez
目标
旨在确定干燥综合征 (SS) 患者小唇唾液腺 (LSG) 的早期浸润中是否存在克隆扩增的 B 细胞。
方法
对 14 名原发性 SS 患者的可用石蜡包埋 LSG 活检进行了研究。通过针对重排的免疫球蛋白基因 DNA 的聚合酶链反应扩增来自 LSG 组织的 DNA。
结果
所有 LSG 标本均显示寡克隆或单克隆 B 细胞扩增。在一名浆细胞肿瘤患者中，同一手术中获得的肿瘤和LSG标本显示出不同的免疫球蛋白基因重排。
结论
克隆扩张是原发性 SS 的特征，在未进一步进展为假性淋巴瘤或淋巴瘤的患者的早期 LSG 浸润中普遍发现（活检后平均随访 4.1 年）。这一特征，加上一名患者中发现的 LSG 和 B 细胞肿瘤之间的克隆不一致，表明额外的步骤对于进展为恶性肿瘤至关重要。
OBJECTIVE
To determine whether clonally expanded B cells are present in the early infiltrates of minor labial salivary glands (LSG) of Sjögren's syndrome (SS) patients.
METHODS
Available paraffin-embedded LSG biopsies from 14 patients with primary SS were studied. DNA from LSG tissue was amplified by a polymerase chain reaction directed toward rearranged immunoglobulin gene DNA.
RESULTS
All LSG specimens showed oligoclonal or monoclonal B cell expansion. In one patient with plasma cell neoplasm, tumor and LSG specimens obtained at the same operation displayed different immunoglobulin gene rearrangements.
CONCLUSION
Clonal expansion is characteristic of primary SS, and it is uniformly found in the early LSG infiltrates of patients who do not experience further progression to pseudolymphoma or lymphoma (mean followup 4.1 years after biopsy). This feature, together with the clonal discordance between the LSG and the B cell neoplasm found in one patient, suggests that additional steps are critical for the progression to malignancy.