Identification and expression profile of an alpha-COPI homologous gene (COPA1) involved in high irradiance and salinity stress in Haematococcus pluvialis
Identification and expression profile of an alpha-COPI homologous gene (COPA1) involved in high irradiance and salinity stress in Haematococcus pluvialis
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DOI：
10.1016/j.algal.2017.10.012
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发表时间：
2017-12
影响因子：
5.1
通讯作者：
Q. Luo;Jing Ning;Zhangli Hu;Chaogang Wang
中科院分区：
文献类型：
--
作者：
Q. Luo;Jing Ning;Zhangli Hu;Chaogang Wang
In response to stresses, Haematococcus pluvialis accumulates triacylglycerols (TAGs) and astaxanthin, which are highly positively correlated in cells. Coatomer protein I (COPI) complexes play important roles in protein and lipid transportation between endoplasmic reticulum (ER) and Golgi apparatus. The proposed functions of COPI alpha-subunit (COPA1) were to maintain the integrity of endomembrane system and to regulate cellular physiological activities (such as autophagy, protein sorting, secretory trafficking and lipid metabolism). However, only scarce information has been reported on this subunit. In this study, the full-length cDNA encodingH. pluvialisCOPA1 (HpCOPA1) and its promoter were cloned and further analyzed by bioinformatics tools. The predicted length of HpCOPA1 was 1234 amino acids (AAs), including WDR, Coatomer-WDAD and COPI-C motifs. The AA sequence of HpCOPA1 was highly identical (78%–99%) to other algal COPA1 and had a highly conserved WDR domain at the N terminus. The promoter of HpCOPA1 containedcis-elements in relation to light and salt stresses, such as AE-box, MNF1, CCAAT-box and TCA-element. Moreover, real-time qPCR analyses showed that the mRNA level ofHpCOPA1was strongly induced by treatments with either high irradiance, or high salinity (45 mM sodium acetate) or in combination.HpCOPA1revealed a delayed but higher peak expression level in treatments with high salinity (12.5-fold at 4 h) compared with high irradiance (4.7-fold at 2 h). To investigate its subcellular localization, HpCOPA1 was expressed in tobacco lower epidermal leaf cells in fusion with GFP. The results showed that HpCOPA1 was localized to plasma membrane and endomembrane system, partly similar to the localization of COPA1 in higher plants and animals. Taken together, the present results would contribute to the further elucidation of the regulatory mechanisms underlying the biosynthesis and transportation of lipids and astaxanthin inH. pluvialis.
