Maintenance of the engrailed expression pattern by Polycomb group genes in Drosophila.
Maintenance of the engrailed expression pattern by Polycomb group genes in Drosophila.
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发表时间：
1992-11
期刊：
影响因子：
4.6
通讯作者：
D. Moazed;P. O’Farrell
中科院分区：
文献类型：
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作者：
D. Moazed;P. O’Farrell
同型基因表达模式的稳定维持依赖于一些负反式调节因子的功能，这些负反式调节因子被称为Polycomb （Pc）基因群。我们已经检查了果蝇片段极性基因的表达模式，在胚胎突变体中为Pc组的几个不同成员嵌入（en）。本研究报告了两个或多个Pc组基因突变的胚胎表现出强烈的异位en表达，而单个Pc组基因突变的胚胎中en只出现微弱的表达下调。这种抑制独立于两个已知的en表达激活因子：en本身和无翅。此外，与在异性梳子- (esc-)突变胚胎中观察到的强异位表达相反，en的表达模式在esc-胚胎中几乎是正常的。这表明esc基因产物在独立于该组其他基因的途径中起作用。这些数据表明，同一组基因需要稳定地限制en表达到条纹模式，以及限制沿前后轴的同型基因的表达，并支持Pc组基因在稳定抑制发育选择基因活性方面的全局作用。
The stable maintenance of expression patterns of homeotic genes depends on the function of a number of negative trans-regulators, termed the Polycomb (Pc) group of genes. We have examined the pattern of expression of the Drosophila segment polarity gene, engrailed (en), in embryos mutant for several different members of the Pc group. Here we report that embryos mutant for two or more Pc group genes show strong ectopic en expression, while only weak derepression of en occurs in embryos mutant for a single Pc group gene. This derepression is independent of two known activators of en expression: en itself and wingless. Additionally, in contrast to the strong ectopic expression of homeotic genes observed in extra sex combs- (esc-) mutant embryos, the en expression pattern is nearly normal in esc- embryos. This suggests that the esc gene product functions in a pathway independent of the other genes in the group. The data indicate that the same group of genes is required for stable restriction of en expression to a striped pattern and for the restriction of expression of homeotic genes along the anterior-posterior axis, and support a global role for the Pc group genes in stable repression of activity of developmental selector genes.