MAPPING OF GENES FOR INHIBIN SUBUNIT-ALPHA, SUBUNIT-BETA-A, AND SUBUNIT-BETA-B ON HUMAN AND MOUSE CHROMOSOMES AND STUDIES OF JSD MICE
MAPPING OF GENES FOR INHIBIN SUBUNIT-ALPHA, SUBUNIT-BETA-A, AND SUBUNIT-BETA-B ON HUMAN AND MOUSE CHROMOSOMES AND STUDIES OF JSD MICE
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DOI：
10.1016/0888-7543(89)90091-8
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发表时间：
1989-07-01
期刊：
影响因子：
4.4
通讯作者：
FRANCKE, U
中科院分区：
文献类型：
--
作者：
BARTON, DE;YANGFENG, TL;FRANCKE, U
抑制素(INH)是一种调节脑垂体FSH分泌的性腺糖蛋白激素，也可能在雄激素的生物合成中发挥作用。有两种形式的抑制素强烈抑制脑下垂体促卵泡激素的分泌。它们共享相同的阿尔法。这个亚基与两个不同的β亚基中的一个共价连接。亚基(βA或βB)。然而，两个β的二聚体。亚基是体外合成和释放卵泡刺激素的有效刺激因子。测试版。亚基与转化生长因子β有广泛的序列相似性。最近，通过对体细胞杂交DNA的Southern杂交分析和原位杂交，已经分离到所有三个抑制素亚单位的cDNA，并将它们的同源基因定位在人和小鼠的染色体上。印度阿尔法。和INH.beta.B基因定位于人类2号染色体Q33.fwdarw区域。QTER和cen.fwdarw。将INHβA基因定位于人染色体7p15.fwdarw。P14是小鼠的13号染色体。小鼠1号染色体上携带其他已知在人类2q染色体上有同源基因的区域包括JSD基因座(用于幼年精原细胞的枯竭)。成年JSD/JSD小鼠血清FSH水平升高，其睾丸中没有精原细胞。JSD中的突变可能与INHα有关。或对JSD/JSD小鼠的INH.beta.B基因进行Southern blotting检测，未检测到大的缺失或重排。
Inhibin (INH) is a gonadal glycoprotein hormone that regulates pituitary FSH secretion and may also play a role in the regulation of androgen biosynthesis. There are two forms of inhibin that strongly inhibit pituitary FSH secretion. These share the same .alpha. subunit this is covalently linked to one of two distinct .beta. subunits (.beta.A or .beta.B). However, dimers of two .beta. subunits are potent stimulators of FSH synthesis and release in vitro. The .beta. subunits share extensive sequence similarity with transforming growth factor .beta.. Recently isolated cDNAs for all three inhibin subunits have been used to map their cognate loci on human and mouse chromosomes by Southern blot analysis of somatic cell hybrid DNAs and by in situ hybridization. INH.alpha. and INH.beta.B genes were assigned to human chromosome 2, regions q33 .fwdarw. qter and cen .fwdarw. q13, respectively, and to mouse chromosome 1. The INH.beta.A locus was mapped to human chromosome 7p15 .fwdarw. p14 an mouse chromosome 13. The region of mouse chromosome 1 that carries other genes known to have homologs on human chromosome 2q includes the jsd locus (for juvenile spermatogonial depletion). Adult jsd/jsd mice have elevated levels of serum FSH and their testes are devoid of spermatogonial cells. The possibility that the mutation in jsd involves the INH.alpha. or INH.beta.B gene was investigated by Southern blotting of DNA from jsd/jsd mice, and no major deletions or rearrangements were detected.