Problems and pitfalls in acetaldehyde determinations.
Problems and pitfalls in acetaldehyde determinations.
复制标题
乙醛测定中的问题和陷阱。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
C. J. Peter Eriksson
中科院分区：
文献类型：
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作者：
C. J. Peter Eriksson
在现有的乙醛分析方法中，各种形成和消失反应使生物样品中乙醛的测定变得复杂。在组织制剂的脱蛋白上清中乙醛的形成（乙醇氧化）是通过使用硫脲来防止的。然而，在脱蛋白过程中，它不受硫脲的抑制，这仍然是血液乙醛测定中的主要问题。为了避免这个问题，建议使用校正曲线，该曲线是通过将对照血液样本添加到脱蛋白剂中产生的，从而使对照血液的稀释度、温度和乙醇浓度（影响人工乙醛形成的主要因素）与样品相同。消失反应主要包括由于结合和/或代谢导致的乙醛损失。这个问题似乎在人类血液样本中很明显，建议快速（少于5秒）去蛋白化。
The determination of acetaldehyde in biologic samples is complicated by a variety of formation and disappearance reactions occurring in the present methods of acetaldehyde analyses. The acetaldehyde formation (ethanol oxidation) in deproteinized supernatant of tissue preparations is prevented by the use of thiourea. During deproteinization, however, it is not inhibited by thiourea, and this remains the main problem in blood acetaldehyde determinations. To circumvent this problem, the use of a correction curve is proposed which is generated by adding control blood samples to the deproteinizing agent such that the blood dilution, temperature, and the ethanol concentrations (the main factors affecting the artifactual acetaldehyde formation) in the controls are identical to those of the samples. Disappearance reactions mainly include loss of acetaldehyde due to binding and/or metabolism. The problem seems to be pronounced with human blood samples, and it is recommended that they be rapidly ( less than 5 sec) deproteinized.
呼吸乙醛：口咽微生物区系和肺微粒体产生乙醛的证据。
影响因子：
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作者：
Pikkarainen,P;Baraona,E;Seitz,H;Lieber,CS
通讯作者：
Lieber,CS