Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea.
Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea.
复制标题
鉴定从小鼠角膜中的循环骨髓来源细胞分化而来的角膜细胞样细胞。
DOI：
10.1007/s00795-013-0031-2
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发表时间：
2013
期刊：
影响因子：
1.8
通讯作者：
Fukushima A.
中科院分区：
文献类型：
--
作者：
Harada Y;Ishida W;Fukuda K;Sumi T;Kawakita T;Taguchi O;Fukushima A.
骨髓（BM）源性干细胞具有分化为多种组织驻留细胞谱系的潜力。已在小鼠角膜中检测到BM衍生的细胞，但发现这些细胞中的大多数是CD 45+或CD 11b+免疫活性细胞。虽然角膜中的一些BM衍生细胞对这些细胞表面标记物呈阴性，但仍不清楚BM来源的细胞是否可以分化为角膜驻留细胞。为了解决这一问题，我们对暴露于致死剂量辐射的野生型小鼠静脉注射来自绿色荧光蛋白（GFP）转基因小鼠的BM细胞。细胞移植后两个月，荧光显微镜显示在整个角膜中存在大量GFP+细胞，在角膜缘区域周围明显存在强烈的GFP荧光。角膜横截面的免疫组织荧光分析显示，大多数BM衍生的GFP+细胞表达CD 45或CD 11b，尽管少数GFP+细胞对这些标记物呈阴性。然而，从角膜基质分离的单个细胞的免疫染色显示，小比例（~ 1%）的GFP+ BM衍生的细胞表达角蛋白细胞特异性蛋白聚糖角蛋白聚糖。我们的研究结果表明，骨髓来源的细胞静脉内引入能够分化成角膜基质的居民细胞。
Bone marrow (BM)-derived stem cells have the potential to differentiate into multiple lineages of tissue resident cells. BM-derived cells have been detected in the mouse cornea, but most of these cells were found to be CD45+or CD11b+immunocompetent cells. Although some BM-derived cells in the cornea were negative for these cell surface markers, it has remained unclear whether cells of BM origin can differentiate into corneal resident cells. To address this issue, we subjected wild-type mice that had been exposed to a lethal dose of radiation to intravenous injection with BM cells from green fluorescent protein (GFP) transgenic mice. Two months after cell transplantation, fluorescence microscopy revealed the presence of numerous GFP+cells throughout the cornea, with intense GFP fluorescence being apparent around the limbal region. Immunohistofluorescence analysis of corneal cross-sections revealed that most of the BM-derived GFP+cells expressed CD45 or CD11b, although a few GFP+cells were negative for these markers. Immunostaining of individual cells isolated from the corneal stroma, however, showed that a small proportion (~1 %) of GFP+BM-derived cells expressed the keratocyte-specific proteoglycan keratocan. Our results suggest that BM-derived cells introduced intravenously are able to differentiate into resident cells of the corneal stroma.