Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and γ-rays irradiations
Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and γ-rays irradiations
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碳离子束和伽马射线辐照拟南芥诱变后代的遗传多态性
DOI：
10.1080/09553002.2020.1688412
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发表时间：
2019-11-15
影响因子：
2.6
通讯作者：
Zhou, Libin
中科院分区：
文献类型：
--
作者：
Chen, Xia;Feng, Hui;Zhou, Libin
目的：重离子束和伽马射线是在高等植物中产生突变的常用物理诱变方法。研究发现，它们具有不同的突变频率和表型诱变谱，但通过与伽玛射线的比较，重离子束的遗传多态特征仍未得到阐明。材料和方法：用碳离子束(线能量转移(LET)为50keV/亩m)和伽马射线(平均LET为0.2keV/亩m)照射拟南芥种子。利用ISSR和随机扩增多态DNA技术，分别对M-1和M-3植株的遗传多样性进行了研究。结果：在M-1和M-3代中，碳离子束诱导的多态率均高于伽马射线：碳离子束辐照的M-1植株的多态率分别为12.87%(ISSR-C)和9.01%(RAPD-C)，而伽马射线诱导的M-1植株的多态率分别为7.67%(ISSR-Gamma)和1.45%(RAPD-Gamma)。在M-3代，ISSR-C、RAPD-C、ISSR-Gamma和RAPD-Gamma的多态频率分别为17.64%、22.79%、12.10%和2.82%。结论：综上所述，碳离子束和伽马射线辐照导致了拟南芥基因组DNA的改变，利用ISSR和RAPD技术可以在M-1和M-3植株上检测到这一变化。因此，碳离子束和伽马射线都能诱导M-1和M-3植株的遗传多态变异。碳离子束诱变的M-1和M-3植株的遗传多态程度均高于伽马射线，说明重离子束诱变育种比常规电离辐射更具优势。M-1植株的平均分子多态比M-3突变体低近4.77%(ISSR-C)、13.78%(RAPD-C)、4.43%(ISSR-Gamma)和1.37%(RAPD-Gamma)。我们希望我们的研究将为理解碳离子束和伽马射线对植物诱变育种的影响提供基础信息。
Purpose: Heavy-ion beams and gamma-rays are popular physical mutagenesis to generate mutations in higher plants. It has been found that they show different mutation frequencies and spectrums of phenotype induction, however, the characteristics of heavy-ion beams on genetic polymorphism have not been clarified by comparing with gamma-rays. Materials and methods: In the present study, seeds of Arabidopsis thaliana were exposed to carbon-ion beams (with linear energy transfer (LET) of 50 keV/mu m) and gamma-rays (with average LET of 0.2 keV/mu m) irradiation. By using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) analysis, the genetic polymorphism of both M-1 and M-3 plants were investigated, respectively. Results: Carbon-ion beams induced relatively higher polymorphism rate in both M-1 and M-3 generation than gamma-rays: the polymorphism rates of M-1 plants derived from carbon-ion beams irradiation are 12.87% (ISSR-C) and 9.01% (RAPD-C), while are 7.67% (ISSR-gamma) and 1.45% (RAPD-gamma) of plants derived from gamma-rays. In M-3 generation, the polymorphism rates of ISSR-C, RAPD-C, ISSR-gamma, and RAPD-gamma are 17.64%, 22.79%, 12.10%, and 2.82%, respectively. Conclusions: In summary, the exposure to carbon-ion beams and gamma-rays lead to the change of genomic DNA of A. thaliana, which could be tested in M-1 plants and M-3 plants by ISSR and RAPD technology. So, both carbon-ion beams and gamma-rays can induce variations of genetic polymorphisms in M-1 plants and M-3 plants. The genetic polymorphisms of M-1 plants and M-3 plants induced by carbon-ion beams are higher than gamma-rays, indicating that heavy-ion beams irradiations mutation breeding is more advantageous than conventional ionizing radiations. Average molecular polymorphism of M-1 plants is lower than M-3 mutants, by nearly 4.77% (ISSR-C), 13.78% (RAPD-C), 4.43% (ISSR-gamma), and 1.37% (RAPD-gamma). We hope our study will provide basic information for understanding the effects of carbon-ion beams and gamma-rays for plant mutation breeding.