Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells
Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells
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DOI：
10.1515/hsz-2012-0258
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发表时间：
2013-02-01
影响因子：
3.7
通讯作者：
Nagata, Koh-ichi
中科院分区：
文献类型：
--
作者：
Mizutani, Yoko;Ito, Hidenori;Nagata, Koh-ichi
我们通过关注人皮肤组织和鳞状细胞癌（SCC）细胞系DJM-1中的SEPT1，对septin进行了生化、组织化学和细胞生物学表征。在免疫印迹中，SEPT1和其他septin在正常人表皮、SCC和DJM-1中检测到。免疫组化分析发现，SEPT1在人表皮细胞和汗腺上皮细胞的细胞质中弥漫性表达，在部分皮肤肿瘤中表达水平升高。在DJM-1细胞中，SEPT1与sept2亚群的其他成员SEPT4和SEPT5在板足中富集，其定位依赖于皮质肌动蛋白结构。在黑色素瘤B16细胞中也观察到SEPT1在板足处的分布。相比之下，SEPT9、SEPT11和SEPT14在DJM-1细胞中与微管一起定位。在免疫沉淀实验中，发现SEPT1和SEPT5在DJM-1细胞中形成一个复合物，而SEPT9、SEPT11和SEPT14与SEPT7、SEPT8和SEPT10等其他SEPT7、SEPT8和SEPT10形成一个不同的复合物，其中SEPT5不包括在内。在DJM-1细胞中，当SEPT1被沉默时，细胞扩散受到抑制。这些结果表明，在DJM-1中，SEPT1可能参与细胞-细胞和/或细胞-底物的相互作用，并与其他septin协同发挥其功能。
We performed biochemical, histochemical and cell biological characterization of septins by focusing on SEPT1 in human skin tissues and a squamous cell carcinoma (SCC) cell line DJM-1. In immunoblotting, SEPT1, together with other septins, was detected in normal human epidermis, SCC and DJM-1. In immunohistochemical analyses, SEPT1 was detected diffusely in the cytoplasm of human epidermal cells and eccrine gland epithelial cells, and the protein level was increased in some skin tumors. In DJM-1 cells, SEPT1 together with other members of SEPT2-subgroup, SEPT4 and SEPT5, was enriched in lamellipodia and the localization was dependent on the cortical actin structure. SEPT1 distribution at lamellipodia was also observed in melanoma B16 cells. SEPT9, SEPT11 and SEPT14, in contrast, were localized along with microtubules in DJM-1 cells. In immunoprecipitation assays, SEPT1 and SEPT5 were found to form a complex in DJM-1 cells, whereas SEPT9, SEPT11 and SEPT14 formed a distinct complex with other septins including SEPT7, SEPT8 and SEPT10, in which SEPT5 was not included. When SEPT1 was silenced in DJM-1 cells, cell spreading was inhibited. These results suggest that SEPT1 may participate in cell-cell and/or cell-substrate interaction in DJM-1 and exert its function in a coordinated manner with other septins.