SWP5, a Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidian Nosema bombycis
SWP5, a Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidian Nosema bombycis
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SWP5（一种孢子壁蛋白）与寄生微孢子虫微孢子虫中的极管蛋白相互作用
DOI：
10.1128/ec.05127-11
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发表时间：
2012-02-01
期刊：
影响因子：
--
通讯作者：
Zhou, Zeyang
中科院分区：
文献类型：
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作者：
Li, Zhi;Pan, Guoqing;Zhou, Zeyang
摘要微孢子虫是一类真核生物胞内寄生虫，可感染几乎所有的脊椎动物和无脊椎动物。微孢子虫的侵入过程包括一个独特的极管进入宿主细胞。孢子壁和极管在微孢子虫的发病机制中发挥着重要作用。迄今为止，已鉴定出5种来自家蚕脑炎原虫和家兔脑炎原虫的孢子壁蛋白（SWP1、SWP2、Enp 1、Enp 2和EcCDA）和5种来自家蚕病原体微孢子虫的孢子壁蛋白（SWP32、SWP30、SWP26、SWP25和NbSWP5）。在这里，我们报告的鉴定和表征的孢子壁蛋白（SWP5）的分子量为20.3 kDa的N。蚕该蛋白与其他真核生物蛋白的序列相似性较低。免疫定位分析表明，SWP5定位于孢子外壁和成熟孢子的极管区域。免疫沉淀，质谱和免疫荧光分析表明，SWP5与极性管蛋白PTP2和PTP3相互作用。用抗SWP5血清预处理成熟孢子，可显著降低孢子的极管挤出率。综上所述，我们的研究结果表明，SWP5是一种定位于孢子壁的孢子壁蛋白，它与极管相互作用，可能在支持孢子壁的结构完整性方面发挥重要作用，并可能调节N.蚕
ABSTRACT Microsporidia are a group of eukaryotic intracellular parasites that infect almost all vertebrates and invertebrates. The microsporidian invasion process involves the extrusion of a unique polar tube into host cells. Both the spore wall and the polar tube play an important role in microsporidian pathogenesis. So far, five spore wall proteins (SWP1, SWP2, Enp1, Enp2, and EcCDA) from Encephalitozoon intestinalis and Encephalitozoon cuniculi and five spore wall proteins (SWP32, SWP30, SWP26, SWP25, and NbSWP5) from the silkworm pathogen Nosema bombycis have been identified. Here we report the identification and characterization of a spore wall protein (SWP5) with a molecular mass of 20.3 kDa in N. bombycis. This protein has low sequence similarity to other eukaryotic proteins. Immunolocalization analysis showed SWP5 localized to the exospore and the region of the polar tube in mature spores. Immunoprecipitation, mass spectrometry, and immunofluorescence analyses revealed that SWP5 interacts with the polar tube proteins PTP2 and PTP3. Anti-SWP5 serum pretreatment of mature spores significantly decreased their polar tube extrusion rate. Taken together, our results show that SWP5 is a spore wall protein localized to the spore wall and that it interacts with the polar tube, may play an important role in supporting the structural integrity of the spore wall, and potentially modulates the course of infection of N. bombycis.