Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): I. Ovine Mx1 is secreted by endometrial epithelial cells via an 'unconventional' secretory pathway.
Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): I. Ovine Mx1 is secreted by endometrial epithelial cells via an 'unconventional' secretory pathway.
复制标题
抗病毒蛋白羊 Mx1 (oMx1) 的细胞定位和功能：I. 羊 Mx1 由子宫内膜上皮细胞通过“非常规”分泌途径分泌。
DOI：
10.1111/j.1600-0897.2006.00444.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Ott,TroyL
中科院分区：
文献类型：
--
作者：
Toyokawa,Koji;Carling,StacyJ;Ott,TroyL
胚胎丢失问题是不孕不育的主要原因。了解导致胚胎丧失的因素将有助于开发提高/调节动物和人类生育能力的技术。研究方法我们检验了抗病毒蛋白绵羊Mx1(OMx1)是由子宫上皮细胞分泌的假设。取周期和早孕母羊的子宫潮红，并检测oMx1蛋白水平。用常规分泌途径的抑制剂布雷菲尔丁A(BFA)测定绵羊腺上皮(OGE)细胞分泌绵羊Mx1的途径。以β2-微球蛋白(β2 MG)作为常规分泌途径的标志物，以干扰素刺激基因15(ISG15)和Galectin-1(Gal-1)作为非常规分泌途径的标志物，观察BFA对绵羊子宫冲洗液中β1蛋白表达的影响。结果绵羊子宫冲洗液中Mx1蛋白水平较低，妊娠15天后表达增加。绵羊GE细胞对干扰素的反应可分泌oMx1，而BFA不能减少oMx1的分泌，提示BFA可减少OMx1的分泌被分泌，并提供了分泌通过非常规分泌途径发生的证据(S)。
ProblemEmbryonic loss is a major contributor to infertility. Understanding factors contributing to embryonic loss will aid in development of technologies to improve/regulate fertility in animals and humans.Method of studyWe tested the hypothesis that the antiviral protein, ovine Mx1 (oMx1), is secreted by uterine epithelial cells. Uterine flushes were obtained from cyclic and early pregnant ewes and examined for levels of oMx1 protein. The pathway for ovine Mx1 secretion in ovine glandular epithelial (oGE) cells was determined using brefeldin A (BFA), an inhibitor of the conventional secretory pathway. Effects of BFA were determined usingβ2‐microglobulin (β2MG) as a marker for the conventional secretory pathway, and interferon stimulated gene 15 (ISG15) and Galectin‐1 (Gal‐1) as markers for the unconventional secretory pathways.ResultsOvine Mx1 protein levels were low in uterine flushes from cyclic ewes and levels increased in pregnant ewes after D 15. Ovine GE cells secreted oMx1 in response to interferon and secretion was not reduced by BFA, suggesting oMx1 was secreted via an unconventional secretory pathway.β2MG secretion was reduced by BFA, whereas ISG15 and Gal‐1 were not.ConclusionThis is the first report that the antiviral protein, oMx1, is secreted and provides evidence that secretion occurs via unconventional secretory pathway(s).