Heterologous expression of geraniol dehydrogenase for identifying the metabolic pathways involved in the biotransformation of citral by Acinetobacter sp. Tol 5
Heterologous expression of geraniol dehydrogenase for identifying the metabolic pathways involved in the biotransformation of citral by Acinetobacter sp. Tol 5
复制标题
香叶醇脱氢酶的异源表达用于鉴定不动杆菌生物转化柠檬醛的代谢途径。
DOI：
10.1080/09168451.2018.1501263
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hori Katsutoshi
中科院分区：
文献类型：
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作者：
Usami Atsushi;Ishikawa Masahito;Hori Katsutoshi
柠檬醛是一种重要的工业单萜类化合物，其生物转化已被广泛研究。但由于柠檬醛是反式异构体香叶醛和顺式异构体橙花醛的混合物，其生物转化的代谢途径尚不清楚。在这里，我们应用了异源表达的geoA，一个基因编码的香叶醇脱氢酶，专门将香叶醇香叶醛和橙花醇橙花醛，以确定参与柠檬醛的生物转化的代谢途径。采用Tol 5来证明该方法的实用性。Tol 5将柠檬醛转化为（1 R，3R，4 R）-1-甲基-4-（1-甲基乙烯基）-1，3-环己二醇和香叶酸。通过表达geoA的Tol 5 β细胞对柠檬醛前体（香叶醇和橙花醇）的生物转化，发现这些化合物是由香叶醛特异性转化而来的。我们的方法，预计将有助于更好地了解参与生物转化的底物是不稳定的，包括几何异构体的代谢途径。
The biotransformation of citral, an industrially important monoterpenoid, has been extensively studied using many microbial biocatalysts. However, the metabolic pathways involved in its biotransformation are still unclear, because citral is a mixture of thetrans-isomer geranial and thecis-isomer neral. Here, we applied the heterologous expression ofgeoA, a gene encoding geraniol dehydrogenase that specifically converts geraniol to geranial and nerol to neral, to identify the metabolic pathways involved in the biotransformation of citral.Acinetobactersp. Tol 5 was employed in order to demonstrate the utility of this methodology. Tol 5 transformed citral to (1R,3R,4R)-1-methyl-4-(1-methylethenyl)-1,3-cyclohexanediol and geranic acid. Biotransformation of citral precursors (geraniol and nerol) by Tol 5 transformant cells expressinggeoArevealed that these compounds were transformed specifically from geranial. Our methodology is expected to facilitate a better understanding of the metabolic pathways involved in the biotransformation of substrates that are unstable and include geometric isomers.