Biological activity of an angiotensin II--ferritin conjugate on rabbit aortic smooth muscle cells.
Biological activity of an angiotensin II--ferritin conjugate on rabbit aortic smooth muscle cells.
复制标题
血管紧张素 II-铁蛋白缀合物对兔主动脉平滑肌细胞的生物活性。
DOI：
10.1021/bi00515a017
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Jamieson,JD
中科院分区：
文献类型：
--
作者：
Schultz,GS;Galardy,RE;Jamieson,JD
方法将血管紧张素与铁蛋白偶联。血管紧张素通过修改制备铁蛋白抗体偶联物的程序与铁蛋白共价偶联（Kishida et al., 1975）。在第一步中,succinicanhydride (2.9 g,更易与29日)添加搅拌1 g的铁蛋白(理论上包含290更易与主要氨基酸组)在75毫升的2 M消息灵通的pH值9和反应在23 C 6 h Succinylated铁蛋白透析过夜在4 C对4 L 1毫米Mes的pH值6,然后应用于1.5×100厘米列交联葡聚糖产物g - 75和筛选了5毫升分数
MethodsCoupling of Angiotensin withFerritin. Angiotensin was covalently coupled to ferritin by using a modification of a procedure for preparing ferritin-antibody conjugates (Kishida et al., 1975). In the first step, succinicanhydride (2.9 g, 29 mmol) was added with stirring to 1 g of ferritin (theoretically containing 290 Mmol of primary amino groups) in 75 mL of 2 M Hepes at pH 9 and reacted for 6 h at 23 C. Succinylated ferritin was dialyzed overnight at 4 C against 4 L of 1 mM Mes at pH 6 and then applied to a 1.5 X 100cm column of Sephadex G-75 equilibrated and eluted in 5-mL fractions with