Anti-psoriasis activities of hydroxytyrosol on HaCaT cells under psoriatic inflammation in vitro
Anti-psoriasis activities of hydroxytyrosol on HaCaT cells under psoriatic inflammation in vitro
复制标题
羟基酪醇对银屑病炎症条件下 HaCaT 细胞的抗银屑病活性
DOI：
10.1080/08923973.2022.2143373
复制
发表时间：
2022-11-09
影响因子：
3.3
通讯作者：
Zhang, Danqun
中科院分区：
文献类型：
--
作者：
Chen, Caifeng;Chen, Li;Zhang, Danqun
银屑病是一种常见的慢性炎症性皮肤病，严重影响患者的生活质量。银屑病的治疗是无法治愈的，迫切需要新的治疗选择。羟基酪醇（HT）具有多种生物活性，如抗炎和抗增殖特性，表明其具有对抗银屑病特征的潜力。然而，其在银屑病的调节中的作用仍然未知。目的探讨HT对银屑病角质形成细胞增殖和炎症反应的影响。方法采用M5细胞因子混合物（包括肿瘤坏死因子（TNF）-α、抑瘤素-M、白细胞介素（IL）-17 A、IL-1 α和IL-22）模拟HaCaT细胞，建立银屑病细胞模型，观察HT对银屑病的体外治疗作用。结果HT通过影响M5诱导的银屑病细胞模型中IL-6、IL-8和TNF-α的表达而发挥抗炎作用。此外，它抑制银屑病角质形成细胞中抗菌蛋白的表达。此外，它抑制银屑病细胞模型中的细胞增殖。结论HT在体外具有抗银屑病作用，可能是一种有前途的治疗银屑病的药物。
Background Psoriasis is a prevalent chronic inflammatory dermatosis, which can significantly impact life quality of patients. The treatment of psoriasis is no cure and novel therapeutic options are urgently needed. Hydroxytyrosol (HT) possesses multiple biological activities, such as anti-inflammatory and anti-proliferation properties, suggesting its potential to counteract hallmarks of psoriasis. However, its role in the regulation of psoriasis remains unknown. Objective In the current study, we explored the anti-proliferative activity and anti-inflammatory responses of HT in psoriatic keratinocytes in vitro. Methods We used M5 cytokines cocktail, which includes tumor necrosis factor (TNF)-alpha, oncostatin-M, interleukin (IL)-17A, IL-1 alpha, and IL-22, to simulate HaCaT cells to establish the cell model of psoriasis and explore the effects of HT on psoriasis in vitro. Results This study showed that HT exerted potent anti-inflammatory effect via influencing the expression of IL-6, IL-8, and TNF-alpha in M5-induced cell model of psoriasis. Moreover, it suppressed the expression of antimicrobial proteins in psoriatic keratinocytes. Additionally, it inhibited cell proliferation in psoriasis cell model. Conclusions Altogether, our results suggested that HT has anti-psoriasis effects in vitro and HT may be a promising therapeutic agent in psoriasis treatment.