Monoclonal antibodies that distinguish antigenic variants of Canine parvovirus
Monoclonal antibodies that distinguish antigenic variants of Canine parvovirus
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DOI：
10.1128/cdli.10.6.1085-1089.2003
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发表时间：
2003-11-01
期刊：
影响因子：
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通讯作者：
Akashi, H
中科院分区：
文献类型：
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作者：
Nakamura, M;Nakamura, K;Akashi, H
犬细小病毒（CPV）被归类为猫细小病毒（FPV）亚群的一员。CPV分离株可分为三种抗原类型：CPV 2型（CPV-2）、CPV-2a和CPV-2b。近年来，从越南豹猫中分离到新的CPV抗原，命名为CPV-2c(a)或CPV-2c(b)。CPV-2c病毒与FPV亚群的其他抗原类型的区别在于与几种单克隆抗体（mab）缺乏反应性。为了表征CPV-2c的抗原性，我们制备了一组针对CPV-2c的单克隆抗体，并通过选择逃逸突变体来检测这些单克隆抗体识别的表位。建立了4种单克隆抗体，并根据反应性将其分为3组：识别CPV-2a、CPV-2b和CPV-2c的单克隆抗体（2G5和20G4）；仅与CPV-2b和CPV-2c(b)反应的单抗（MAb 21C3）；以及识别所有类型的FPV亚群病毒的单克隆抗体（MAb 19D7）。MAb 20G4与CPV-2c的反应性高于与CPV-2a和CPV-2b的反应性。这些类型的单克隆抗体的特异性以前没有报道过。通过对中和抗性突变体的分析进行的定位研究表明，MAb 210和19D7识别的表位属于抗原位点A。B位点和其他抗原位点的残基替换影响MAb 2G5识别的表位。提示MAb 20G4识别的抗原表位与抗原位点b有关，这些MAb可望用于FPV亚群分离物的检测和分类。
Canine parvovirus (CPV) is classified as a member of the feline parvovirus (FPV) subgroup. CPV isolates are divided into three antigenic types: CPV type 2 (CPV-2), CPV-2a, and CPV-2b. Recently, new antigenic types of CPV were isolated from Vietnamese leopard cats and designated CPV-2c(a) or CPV-2c(b). CPV-2c viruses were distinguished from the other antigenic types of the FPV subgroup by the absence of reactivity with several monoclonal antibodies (MAbs). To characterize the antigenicity of CPV-2c, a panel of MAbs against CPV-2c was generated and epitopes recognized by these MAbs were examined by selection of escape mutants. Four MAbs were established and classified into three groups on the basis of their reactivities: MAbs which recognize CPV-2a, CPV-2b, and CPV-2c (MAbs 2G5 and 20G4); an MAb which reacts with only CPV-2b and CPV-2c(b) (MAb 21C3); and an MAb which recognizes all types of the FPV subgroup viruses (MAb 19D7). The reactivity of MAb 20G4 with CPV-2c was higher than its reactivities with CPV-2a and CPV-2b. These types of specificities of MAbs have not been reported previously. A mapping study by analysis of neutralization-resistant mutants showed that epitopes recognized by MAbs 210 and 19D7 belonged to antigenic site A. Substitution of the residues in site B and the other antigenic site influenced the epitope recognized by MAb 2G5. It was suggested that the epitope recognized by MAb 20G4 was related to antigenic site B. These MAbs are expected to be useful for the detection and classification of FPV subgroup isolates.