Generation of Pax2-Cre mice by modification of a Pax2 bacterial artificial chromosome
Generation of Pax2-Cre mice by modification of a Pax2 bacterial artificial chromosome
复制标题
DOI：
10.1002/gene.20017
复制
发表时间：
2004-04-01
期刊：
影响因子：
1.5
通讯作者：
Groves, AK
中科院分区：
文献类型：
--
作者：
Ohyama, T;Groves, AK
Pax 2基因在发育中的耳囊、肾和中脑-后脑边界中表达。我们通过修饰Pax 2细菌人工染色体（BAC）产生Pax 2-Cre转基因株系。在一个Pax 2-Cre系中，Cre mRNA在前体节晚期开始在耳基板中表达。R26 R报告小鼠分析显示，Cre表达足以删除内耳中大多数细胞中的IoxP侧翼序列。在其他Pax 2表达组织如中脑、小脑、嗅球和肾脏中也检测到报告基因阳性细胞，表明这些细胞是这些组织中Pax 2表达细胞的后代，并且Pax 2-Cre转基因小鼠可以有效地删除这些组织中的基因。(C)2004 Wiley-Liss，Inc.
The Pax2 gene is expressed in the developing otocyst, kidney, and midbrain-hindbrain boundary. We generated Pax2-Cre transgenic lines by modification of a Pax2 bacterial artificial chromosome (BAC). In one Pax2-Cre line, Cre mRNA starts to be expressed in the otic placode at the late presomite stage. R26R reporter mouse analysis revealed that the Cre expression is sufficient to delete the IoxP-flanked sequences in most of the cells in the inner ear. Reporter-positive cells are also detected in other Pax2-expressing tissues such as midbrain, cerebellum, olfactory bulb, and kidney, suggesting that these cells are the descendants of Pax2-expressing cells in these tissues and that Pax2-Cre transgenic mice can delete genes efficiently in these tissues. (C) 2004 Wiley-Liss, Inc.