DETECTION OF NEUROBLASTOMA-CELLS IN BLOOD
DETECTION OF NEUROBLASTOMA-CELLS IN BLOOD
复制标题
DOI：
10.1200/jco.1990.8.4.736
复制
发表时间：
1990-04-01
影响因子：
45.3
通讯作者：
SANDERS, DG
中科院分区：
文献类型：
--
作者：
MOSS, TJ;SANDERS, DG
肿瘤监测试验用于检测复发并指导临床医生进行治疗改变。在疾病过程中早期发现复发可能会改善长期生存。对于神经母细胞瘤患者，目前常规的监测方法包括放射学检查、骨髓分析、体格检查和尿儿茶酚胺代谢物测定。血液免疫细胞学分析检测循环肿瘤细胞是一种高度特异性和敏感性的方法，这可能被证明是有用的监测患者与神经母细胞瘤。在我们的研究中，10例已知播散性疾病的患者中有7例在诊断时检测到循环肿瘤细胞，13例患者中有6例在治疗期间检测到循环肿瘤细胞。在一些患者中，在100，000个正常造血细胞中仅识别出两个肿瘤细胞。在治疗期间评估的13例患者中循环神经母细胞的存在或不存在与肿瘤复发显著相关（P = .002）。我们的结论是，血液免疫细胞学分析是一个敏感的方法检测循环神经母细胞。我们建议将这项技术与其他常规方法结合使用，以提高肿瘤检测和神经母细胞瘤患者的后续监测。
Tumor surveillance tests are used to detect relapse and to guide the clinician in making therapeutic changes. Identification of relapse early in the course of disease may improve long-term survival. For patients with neuroblastoma, current conventional surveillance methods include radiologic testing, bone marrow analysis, physical examination, and measurement of urinary catecholamine metabolites. Immunocytologic analysis of blood for the detection of circulating tumor cells is a highly specific and sensitive method, which may prove useful in monitoring patients with neuroblastoma. In our study, circulating tumor cells were detected in seven of 10 patients with known disseminated disease at diagnosis and in six of 13 patients during therapy. In some patients, as few as two tumor cells were identified among 100,000 normal hematopoietic cells. The presence or absence of circulating neuroblasts in the 13 patients evaluated during therapy was significantly correlated with tumor relapse (P = .002). We conclude that immunocytologic analysis of blood is a sensitive method for the detection of circulating neuroblasts. We recommend that this technique be used in conjunction with other conventional methods for improved tumor detection and subsequent monitoring of neuroblastoma patients.