ent-Steroids: Novel tools for studies of signaling pathways
ent-Steroids: Novel tools for studies of signaling pathways
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DOI：
10.1016/j.steroids.2008.11.019
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发表时间：
2009-07-01
期刊：
影响因子：
2.7
通讯作者：
Covey, Douglas F.
中科院分区：
文献类型：
--
作者：
Covey, Douglas F.
膜受体通常受类固醇调节，因此有必要区分类固醇对这些受体的作用与对核受体的作用。此外，区分类固醇与膜受体结合引起的直接效应和类固醇干扰含有受体的膜引起的对膜受体功能的间接效应在机制上也可能是重要的。在这方面，内分泌类固醇，自然发生的类固醇的镜像，是区分类固醇的这些不同作用的新工具。综述提供了一个背景，了解不同的行动，可以预期的类固醇和ent-类固醇在生物系统中，参考的ent-类固醇的制备，简短的讨论有关形式的立体异构和需要满足的要求，两个分子之间的相互作用是对映选择性。然后，综述总结了自1992年以来发表的生物物理，生物化学和药理学研究的结果，其中内分泌类固醇已被用于研究类固醇在膜和/或受体介导的信号通路中的作用。(C)2008年爱思唯尔公司All rights reserved.
Membrane receptors are often modulated by steroids and it is necessary to distinguish the effects of steroids at these receptors from effects occurring at nuclear receptors. Additionally, it may also be mechanistically important to distinguish between direct effects caused by binding of steroids to membrane receptors and indirect effects on membrane receptor function caused by steroid perturbation of the membrane containing the receptor. In this regard, ent-steroids, the mirror images of naturally occurring steroids, are novel tools for distinguishing between these various actions of steroids. The review provides a background for understanding the different actions that can be expected of steroids and ent-steroids in biological systems, references for the preparation of ent-steroids, a short discussion about relevant forms of stereoisomerism and the requirements that need to be fulfilled for the interaction between two molecules to be enantioselective. The review then summarizes results of biophysical, biochemical and pharmacological studies published since 1992 in which ent-steroids have been used to investigate the actions of steroids in membranes and/or receptor-mediated signaling pathways. (C) 2008 Elsevier Inc. All rights reserved.