The effect of statins on mRNA levels of genes related to inflammation, coagulation, and vascular constriction in HUVEC. Human umbilical vein endothelial cells.
The effect of statins on mRNA levels of genes related to inflammation, coagulation, and vascular constriction in HUVEC. Human umbilical vein endothelial cells.
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DOI：
10.5551/jat.9.178
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发表时间：
2002-08
影响因子：
4.4
通讯作者：
S. Morikawa;Wakako Takabe;C. Mataki;T. Kanke;Takahiro Itoh;Y. Wada;A. Izumi;Y. Saito;T. Hamakubo-T.
中科院分区：
文献类型：
--
作者：
S. Morikawa;Wakako Takabe;C. Mataki;T. Kanke;Takahiro Itoh;Y. Wada;A. Izumi;Y. Saito;T. Hamakubo-T.
大规模临床试验表明，他汀类药物治疗后心血管事件显着减少。然而，在这些试验中观察到的他汀类药物治疗的益处可能比单独降低血脂水平所预期的益处更大。为了阐明他汀类药物预防血管壁细胞心血管事件的机制，我们使用DNA微阵列研究了与阿托伐他汀或匹伐他汀一起培养的人脐静脉内皮细胞中基因表达谱的变化。他汀类药物影响炎症、凝血和血管收缩相关基因的表达水平。他汀类药物治疗后，白细胞介素 8 (IL-8) 和单核细胞趋化蛋白 1 (MCP-1) 的 mRNA 水平下降。他汀类药物可降低纤溶酶原激活剂抑制剂-1 (PAI-1) 的 mRNA 水平，并增加血栓调节蛋白的 mRNA 水平。他汀类药物可降低内皮素 1 的 mRNA 水平，并增加一氧化氮合酶 3 (eNOS) 的 mRNA 水平。这些结果表明，他汀类药物在临床上是有效的，因为它们能够改变内皮细胞的基因表达谱，从而预防血管事件。
Large-scale clinical trials have demonstrated significant reductions in cardiovascular events following statin therapy. The observed benefit of statin therapy, however, may be greater in these trials than is to be expected from lowering lipid levels alone. In order to clarify the mechanism by which statins prevent cardiovascular events in vascular wall cells, we investigated the changes in gene expression profiles after incubation with atorvastatin or pitavastatin in cultured human umbilical vein endothelial cells using DNA microarrays. Statins affected the expression levels of genes involved in inflammation, coagulation, and vascular constriction. The mRNA levels for interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) decreased after statin treatment. Statins reduced mRNA levels of plasminogen activator inhibitor-1 (PAI-1) and increased the mRNA levels of thrombomodulin. Statins reduced the mRNA levels of endothelin-1 and increased the mRNA levels of nitric oxide synthase-3 (eNOS). These results show that, statins are clinically effective because of their ability to change the gene expression profile of endothelial cells thereby preventing vascular events.