Biochemical methods to assess CFTR expression and membrane localization.
Biochemical methods to assess CFTR expression and membrane localization.
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DOI：
10.1016/j.jcf.2004.05.017
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Amaral, Margarida D
中科院分区：
文献类型：
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作者：
Farinha, Carlos M;Penque, Deborah;Amaral, Margarida D
囊性纤维化跨膜传导调节蛋白（CFTR）的检测通常是一项困难的任务，因为该膜蛋白在细胞中呈低水平表达和高周转率。常用的生化方法可用于CFTR的检测，但必须考虑几个关键点。本文的范围是概述常用的生化方法来评估CFTR的表达，加工和膜定位。
Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.