Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells
Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells
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DOI：
10.1016/j.ygeno.2018.05.007
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发表时间：
2019-07-01
期刊：
影响因子：
4.4
通讯作者：
Masoudi-Nejad, Ali
中科院分区：
文献类型：
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作者：
Dehghanian, Fariba;Hojati, Zohreh;Masoudi-Nejad, Ali
Hippo信号通路是一种潜在的调控通路，在干细胞的分化和自我更新中起着关键作用。Yap1是该途径的主要转录效应因子。Yap1在胚胎干细胞（ESCs）和分化过程中的重要性仍然是一个具有挑战性的问题，因为有两种不同的观察结果被报道。为了回答这个问题，我们使用了共表达网络和差异共表达分析，然后进行了实验验证。我们的研究结果表明，Yap1在ESCs中与干细胞标记物高度共表达，而在分化细胞（DCs）中则没有。在P19分化过程中，Yap1显著下调，Yap1易位到细胞质中。此外，我们的结果表明E2f7、Lin28a和Dppa4基因可能是干细胞Hippo通路的调控核因子。目前的研究结果与表明Yap1是干细胞自我更新的重要因子的研究积极一致。
The Hippo signaling pathway is identified as a potential regulatory pathway which plays critical roles in differentiation and stem cell self-renewal. Yap1 is a primary transcriptional effector of this pathway. The importance of Yap1 in embryonic stem cells (ESCs) and differentiation procedure remains a challenging question, since two different observations have been reported. To answer this question we used co-expression network and differential co-expression analyses followed by experimental validations. Our results indicate that Yap1 is highly co-expressed with stem cell markers in ESCs but not in differentiated cells (DCs). The significant Yap1 downregulation and also translocation of Yap1 into the cytoplasm during P19 differentiation was also detected. Moreover, our results suggest the E2f7, Lin28a and Dppa4 genes as possible regulatory nuclear factors of Hippo pathway in stem cells. The present findings are actively consistent with studies that suggested Yap1 as an essential factor for stem cell self-renewal.