Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects
Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects
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DOI：
10.1097/mcg.0b013e3182693982
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发表时间：
2012-10-01
影响因子：
2.9
通讯作者：
Mogna, Giovanni
中科院分区：
文献类型：
--
作者：
Drago, Lorenzo;Toscano, Marco;Mogna, Giovanni
目标：本研究的目的是表征肠道菌群在健康的百岁老人与年轻的成年人相比，定量和定性方面的肠道群落structure.Background：肠道菌群在人类健康中起着至关重要的作用。至于资历，其平衡受到深层生理变化的影响。长寿的人（年龄> 90岁）具有不同于年轻老年人的不寻常特征，因此在分析与年龄相关的特征时应单独考虑。然而，他们已经被纳入少数研究，他们通常被分组在一起，与年轻的老年人。研究：肠道菌群的14个百岁老人和10个年轻的成年人进行了分析。列举了属于以下组的可培养细菌：肠杆菌科、肠球菌属、葡萄球菌属、乳杆菌属、双歧杆菌属、梭菌属、拟杆菌属和酵母。结果：在百岁老人中，肠杆菌科、双歧杆菌和类杆菌数量减少，狭义梭菌数量增加（P < 0.05）。在百岁老人和年轻人中分离出的乳杆菌和双歧杆菌的数量是相似的。乳杆菌亚群的组成在组之间是相当不同的。肠道中长双歧杆菌的存在似乎是百岁老人的一个特殊特征。有趣的是，仅1株B.结论：百岁老人的肠道菌群具有不同于年轻人和年轻老年人的特殊特征。进一步的研究将有助于了解肠道微生物群是否会影响预期寿命，以及益生菌的施用是否有助于延长人类寿命。
Goal: The aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure.Background: The gut microbiota plays an essential role in human health. Toward seniority, its balance is affected by deep physiological changes. Long-lived people (age > 90 y) have unusual features that differ from the younger elderly, so they should be considered separately when analyzing age-related features. However, they have been included in few studies and they have usually been grouped together with the younger elderly.Study: The gut microbiota of 14 centenarians and 10 younger adults was analyzed. Cultivable bacteria belonging to the following groups were enumerated: enterobacteriaceae, Enterococcus, Staphylococcus, Lactobacillus, Bifidobacterium, Clostridium, Bacteroides, and yeast. Lactobacilli and Bifidobacteria were further characterized at the species level by pyrosequencing.Results: In centenarians, we observed a reduction in the quantity of enterobacteriaceae, bifidobacteria, and bacteroides and an increase in clostridia sensu stricto (P < 0.05). The number of Lactobacillus and Bifidobacterium species isolated in centenarians and younger adults was similar. The composition of the Lactobacillus subpopulation was quite different between the groups. The presence of Bifidobacterium longum in the gut seems to be a particular feature in centenarians. It is interesting to note that only 1 strain of B. longum was isolated from each centenarian subject.Conclusions: The gut microbiota of centenarians has particular features that differ from both younger adults and the younger elderly. Further studies would help to understand whether the intestinal microbiota can influence life expectancy and whether the administration of probiotic bacteria could help to extend the longevity of human life.