Insulin-like growth factor-1 induces lymphangiogenesis and facilitates lymphatic metastasis in colorectal cancer
Insulin-like growth factor-1 induces lymphangiogenesis and facilitates lymphatic metastasis in colorectal cancer
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DOI：
10.3748/wjg.v19.i43.7788
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发表时间：
2013-11-21
影响因子：
4.3
通讯作者：
Zhou, Shu-Zhen
中科院分区：
文献类型：
--
作者：
Li, Zhen-Jun;Ying, Xiao-Jiang;Zhou, Shu-Zhen
目的：目的：探讨胰岛素样生长因子-1（IGF-1）及其受体（IGF-1 R）在结直肠癌（CRC）组织中的表达及其与淋巴管生成和淋巴结转移的关系。分析IGF-1/IGF-1 R与LVD的相关性。采用transwell小室法检测IGF-1对大肠癌细胞迁移和侵袭的影响。结果：IGF-1和IGF-1 R在大肠癌组织中的表达与淋巴结转移（r = 0.715和0.569，P < 0.05）和肿瘤TNM分期（r = 0.731和0.609，P < 0.05）相关;结直肠癌组织中LVD也明显增高，且与淋巴结转移有关（r = 0.405，P < 0.05）。LVD与IGF 1 R表达呈正相关（r = 0.437，P < 0.05）。Transwell实验显示IGF-1增加了CRC细胞的迁移和侵袭。结论：IGF-1/IGF-1 R信号通路可诱导肿瘤相关淋巴管生成，促进结直肠癌淋巴道转移。(C)2013百世登出版集团有限公司有限公司All rights reserved.
AIM: To investigate the expression of insulin-like growth factor-1 (IGF-1)/insulin-like growth factor-1 receptor (IGF-1R) in colorectal cancer (CRC) tissues and to analyze their correlation with lymphangiogenesis and lymphatic metastasis.METHODS: Immunohistochemistry was used to evaluate IGF-1 and IGF-1R expression and lymphatic vessel density (LVD) in 40 CRC specimens. The correlation between IGF-1/IGF-1R and LVD was investigated. Effects of IGF-1 on migration and invasion of CRC cells were examined using transwell chamber assays. A LoVo cell xenograft model was established to further detect the role of IGF-1 in CRC lymphangiogenesis in vivo.RESULTS: Elevated IGF-1 and IGF-1R expression in CRC tissues was correlated with lymph node metastasis (r = 0.715 and 0.569, respectively, P < 0.05) and tumor TNM stage (r = 0.731 and 0.609, P < 0.05). A higher LVD was also found in CRC tissues and was correlated with lymphatic metastasis (r = 0.405, P < 0.05). A positive correlation was found between LVD and IGF1R expression (r = 0.437, P < 0.05). Transwell assays revealed that IGF-1 increased the migration and invasion of CRC cells. In vivo mouse studies showed that IGF-1 also increased LVD in LoVo cell xenografts.CONCLUSION: IGF-1/IGF-1R signaling induces tumor-associated lymphangiogenesis and contributes to lymphatic metastasis of CRC. (C) 2013 Baishideng Publishing Group Co., Limited. All rights reserved.