INHIBITION OF RAT ARTERIAL SMOOTH-MUSCLE CELL-PROLIFERATION BY HEPARIN .2. INVITRO STUDIES
INHIBITION OF RAT ARTERIAL SMOOTH-MUSCLE CELL-PROLIFERATION BY HEPARIN .2. INVITRO STUDIES
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DOI：
10.1161/01.res.47.4.578
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发表时间：
1980-01-01
影响因子：
20.1
通讯作者：
KARNOVSKY, MJ
中科院分区：
文献类型：
--
作者：
HOOVER, RL;ROSENBERG, R;KARNOVSKY, MJ
本文研究了肝素对体外培养的大鼠主动脉平滑肌细胞生长的影响。通过[~3H]胸腺嘧啶核苷摄取量和细胞数在3天内的变化来监测细胞生长情况。肝素高度抗凝或非抗凝，显著抑制血管平滑肌细胞的生长。就分子和细胞类型而言，这是一种高度特异的相互作用：即，除了低分子质量的葡聚糖硫酸盐外，其他聚阴离子对生长的影响并不相同，并不是所有的细胞都被肝素抑制；例如，内皮细胞的生长实际上得到了促进。在从介质中去除抗凝血酶后，实验表明，即使凝血酶这种强有力的有丝分裂原仍然存在并具有活性，肝素仍是有效的。将血小板提取液通过肝素柱并不能去除血小板制剂的所有促有丝分裂活性。这两个实验都表明了肝素分子的抑制作用。肝素可通过抑制大鼠颈动脉损伤后的平滑肌细胞增殖而限制大鼠颈动脉损伤后肌内膜斑块的大小。
The effects of heparin on the growth of rat aortic smooth muscle cells were studied in vitro. Measurements of growth were monitored by [3H]thymidine uptake and changes in cell number over 3 days. Heparin highly anticoagulant or nonanticoagulant, significantly inhibits growth of smooth muscle cells. This is a highly specific interaction with regard to molecule and cell type: i.e., other polyanions, except for a low molecular weight dextran sulfate, do not have the same effect on growth and not all cells are inhibited by heparin; e.g., endothelial cell growth actually is enhanced. After removing antithrombin from the media, experiments showed that heparin is effective even though thrombin, a potent mitogenic agent, is still present and active. Passing the platelet extract over a heparin column did not remove all mitogenic activity of the platelet preparation. Both experiments indicate an inhibitory role for the heparin molecule. Heparin can limit the size of myointimal plaques in rats after carotid injuries by inhibiting smooth muscle cell proliferation.