Alkanethiol containing glycopolymers: a tool for the detection of lectin binding.
Alkanethiol containing glycopolymers: a tool for the detection of lectin binding.
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DOI：
10.1016/j.bmcl.2007.08.002
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发表时间：
2007-10
影响因子：
2.7
通讯作者：
Mingchuan Huang;Zhihong Shen;Yalong Zhang;Xiangqun Zeng;P. Wang
中科院分区：
文献类型：
--
作者：
Mingchuan Huang;Zhihong Shen;Yalong Zhang;Xiangqun Zeng;P. Wang
糖共聚物是具有非碳水化合物主链的有用的大分子，用于以多价形式呈现糖。在此，通过用含胺单体取代预活化的聚[N-（丙烯酰氧基）琥珀酰亚胺]（pNAS），合成了含有甘露糖和烷基乙氧基连接基的糖共聚物。利用所得到的糖基共聚物，通过烷基乙氧基化官能团的自组装策略，在石英晶体微天平（QCM）的金表面生成了糖表面。此外，所得到的糖表面用于检测甘露糖特异性凝集素伴刀豆球蛋白A（Con A）的结合。
Glycopolymers are useful macromolecules with a non-carbohydrate backbone for presenting saccharides in multivalent form. Here, glycopolymers containing mannose and alkanethiol linker were synthesized through substituting preactivated poly [N-(acryloyloxy) succinimide] (pNAS) with amine-containing monomer. With the obtained glycopolymers, a glycosurface was generated on the gold surface of quartz crystal microbalance (QCM) through self-assembled strategy by the use of alkanethiol functional group. Furthermore, the resulting glycosurface was used to detect the binding of mannose specific lectin concanavalin A (Con A).