Identification of Arabidopsis thaliana variants with differential glyphosate responses
Identification of Arabidopsis thaliana variants with differential glyphosate responses
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DOI：
10.1016/j.jplph.2006.08.008
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Widholm, Jack M.
中科院分区：
文献类型：
--
作者：
Brotherton, Jeffrey E.;Jeschke, Mark R.;Widholm, Jack M.
采用方法获得草甘膦应答不同的拟南芥变体。用哥伦比亚-0幼苗进行了通过喷洒亚致死浓度草甘膦的轮回选择。在含有0.2mM草甘膦的琼脂上筛选了800，000个甲基磺酸乙酯诱变的M-2幼苗群体，该浓度低于先前在其他研究中使用的浓度，并且没有回收到抗性突变体。用草甘膦筛选了72个拟南芥生态型，并观察到一系列反应。在对这些生态类型的一个子集进行的后续实验中，相对于未处理的对照，在生态类型中，草甘膦使种子产量减少11.5克/公顷（约为典型田间使用率的11%），范围从0%到> 90%。然而，即使是最不敏感的生态类型也受到相对较低的台风率的严重伤害。总体而言，试图选择拟南芥幼苗，显着草甘膦抗性是不成功的，与以前的报告一致。虽然拟南芥对草甘膦的敏感性限制了其在草甘膦抗性机制研究中的应用，但具有不同抗草甘膦反应的拟南芥生态型可用于进一步的研究。(C)2006年Elsevier GrnbH。All rights reserved.
approaches were taken to obtain Arabidopsis thaliana variants that differed in glyphosate response. Recurrent selection by spraying with sub-lethal glyphosate concentrations was performed with Columbia-0 seedlings. After seven cycles of treatment, no resistance was found. A population of 800,000 ethylmethanesulfonate-mutagenized M-2 seedlings was screened on agar containing 0.2 mM glyphosate, a lower concentration than that previously used in other studies, and no resistant mutants were recovered. Seventy-two Arabidopsis ecotypes were screened with glyphosate and a range of responses was observed. In a follow-up experiment on a subset of these ecotypes, reduction of seed yield by 11.5 g/ha glyphosate (about 11% the typical field use rate) ranged among ecotypes from 0% to >90%, relative to untreated controls. However, even the least sensitive ecotypes were severely injured by relatively tow gtyphosate rates. Overall, attempts to select Arabidopsis seedlings that were significantly glyphosate-resistant were unsuccessful and consistent with previous reports. Arabidopsis ecotypes identified with differential gtyphosate responses could be used for further studies though the inherently high sensitivity of Arabidopsis to gtyphosate could limit their utility in studying glyphosate-resistance mechanisms. (C) 2006 Elsevier GrnbH. All rights reserved.