The conservation genomics of the endangered distylous gypsophile Oreocarya crassipes (Boraginaceae)
The conservation genomics of the endangered distylous gypsophile Oreocarya crassipes (Boraginaceae)
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DOI：
10.1007/s10592-019-01212-1
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发表时间：
2019-08
影响因子：
2.2
通讯作者：
J. Cohen
中科院分区：
文献类型：
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作者：
J. Cohen
奇瓦瓦沙漠包括许多特有的被子植物物种，其中一些具有非常有限的地理范围。其中一个种是厚壳山核桃（Oreocarya crassipes（I. M. Johnst.）哈森斯塔布公司G. Simpson，一种濒危的二端石吊兰，产于美国得克萨斯州布鲁斯特县南部的外佩科斯地区。该物种已知有10个种群，而这一少数种群、该地区的人类发展、二端繁殖系统和土壤要求威胁着该物种的长期生存能力。使用数百个单核苷酸多态性，通过可调基因分型测序（tGBS）和10个微卫星位点，遗传多样性，人口，选择和迁移模式进行了检查，从4个群体的192个ofO。粗壮的从抽样的个体中，通过多种方法和两种类型的数据确定了两个群体（集群）。利用SNP数据，在这些群体中的一个群体中进一步解析群体子结构，以识别两个不同的个体群体。多个被认为具有混合血统的个体，沿着Fst值和AMOVA结果，提供了种群之间遗传交换的证据，这种情况在吉普赛人中比非吉普赛人中不太常见，而且种群之间的迁移率最近一直在增加。Fst值为O. crassipes更类似于其他稀有物种比其他gypsophiles。此外，虽然distyly具体不一定影响种群遗传学的物种，异花受精，这是促进distyly，似乎发挥了作用的遗传结构ofO。粗壮的
The Chihuahuan Desert includes many endemic angiosperm species, some having very restricted geographic ranges. One of these species isOreocarya crassipes(I. M. Johnst.) Hasenstab & M. G. Simpson, an endangered distylous gypsophile from the Trans-Pecos region in southern Brewster County, Texas, USA. The species is known from 10 populations, and this small number of populations, human development in the area, a distylous breeding system, and edaphic requirements threaten the long-term viability of the species. Using both hundreds of single nucleotide polymorphisms identified via tunable genotyping-by-sequencing (tGBS) and 10 microsatellite loci, patterns of genetic diversity, demography, selection, and migration were examined for 192 individuals from four populations ofO. crassipes. From the sampled individuals, two populations (clusters) were identified via multiple methodologies and with both types of data. With SNP data, population substructure was further resolved among one of these populations to identify two distinct groups of individuals. Multiple individuals recognized as having mixed ancestry, along withFst values and AMOVA results, provide evidence of genetic exchange among populations, which is less common for gypsophiles than non-gypsophiles, and the rate of migration among populations has been increasing recently. TheFst values forO. crassipesare more similar to those of other rare species than to other gypsophiles. Additionally, while distyly specifically does not necessarily impact the population genetics of the species, allogamy, which is facilitated by distyly, seems to have played a role in the genetic structure ofO. crassipes.