Predicting hepatocellular carcinoma development for cirrhosis patients via methylation detection of heparocarcinogenesis-related genes
Predicting hepatocellular carcinoma development for cirrhosis patients via methylation detection of heparocarcinogenesis-related genes
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DOI：
10.7150/jca.24024
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发表时间：
2018-01-01
影响因子：
3.9
通讯作者：
Qin, Yang
中科院分区：
文献类型：
--
作者：
Huang, Yuan;Wei, Ling;Qin, Yang
背景资料：大多数肝细胞癌（HCC）患者经历了从慢性肝炎，然后肝硬化（LC），最后到癌的进展。本研究的目的是阐明预测肝硬化患者发生肝癌的危险因素。应用多重甲基化特异性PCR（MSP）检测396例肝硬化患者肝组织和血浆中肝癌发生相关基因的甲基化状态，并应用多变量cox模型分析肝硬化患者肝癌发生与危险因素的关系。结果：105例LC患者术后30个月肝癌发生率为30.48%（32/105），肝癌发生率与患者年龄、p16、SFRP、LINE1基因异常甲基化有关（P
Background: Most hepatocellular carcinoma (HCC) patients have undergone a progression from chronic hepatitis, then liver cirrhosis (LC), and finally to carcinoma. The objective of this study was to elucidate risk factors to predict HCC development for cirrhosis patients.Methods: Multiple methylated specific PCR (MSP) was applied to determine methylation status of heparocarcinogenesis-related genes in 396 tissue and plasma specimens and multivariate cox model was used to analyze the relationship between risk variables and HCC development among cirrhosis patients, followed up in a median period of 30 months.Results: Among 105 LC cases, HCC incidence rate at 30 months was 30.48% (32/105), which were statistically associated with patients' age and aberrant methylation of p 16, SFRP, and LINE1 (p