Capabilities of the WinLTP data acquisition program extending beyond basic LTP experimental functions
Capabilities of the WinLTP data acquisition program extending beyond basic LTP experimental functions
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DOI：
10.1016/j.jneumeth.2006.12.018
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发表时间：
2007-05-15
影响因子：
3
通讯作者：
Collingridge, Graham L.
中科院分区：
文献类型：
--
作者：
Anderson, William W.;Collingridge, Graham L.
WinLTP是一款Windows数据采集程序，专为研究长时程增强（LTP）、长时程抑制（LTD）以及一般的突触反应而设计。基本的LTP和LTD实验所需的功能包括交替的双输入细胞外通路刺激、单串刺激、θ节律刺激和引发爆发刺激诱导LTP，以及低频刺激诱导LTD。WinLTP提供对突触波形的在线分析，包括斜率、峰值幅度、群体峰电位幅度、平均幅度、面积、上升时间、衰减时间、持续时间、细胞输入电阻和串联电阻的测量。WinLTP还具有许多超越基本LTP实验功能的高级能力：（1）在一次扫描中对所有诱发的突触电位分别进行分析，并将整个串刺激诱发的突触反应作为一个单一实体进行分析。（2）多任务处理可用于运行连续采集任务（将数据保存到无间隙的Axon二进制文件），同时运行刺激/采集扫描任务。（3）动态协议脚本可用于制定更复杂的协议，涉及嵌套循环（带计数器）、延迟、扫描（带各种刺激）和运行函数（执行一组函数）。在实验运行过程中可以改变协议流程。WinLTP可在美国国家仪器公司的M系列和分子器件公司的Digidata 132x板上运行，可从www.winltp.com获取。（C）2007爱思唯尔公司。保留所有权利。
WinLTP is a Windows data acquisition program designed for the investigation of long-term potentiation (LTP), long-term depression (LTD), and synaptic responses in general. The capabilities required for basic UP and LTD experiments include alternating two-input extracellular pathway stimulation, UP induction by single train, theta burst, and primed burst stimulation, and LTD induction by low frequency stimulation. WinLTP provides on-line analyses of synaptic waveforms including measurement of slope, peak amplitude, population-spike amplitude, average amplitude, area, rise time, decay time, duration, cell input resistance, and series resistance. WinLTP also has many advanced capabilities that extend beyond basic LTP experimental capabilities: (1) analysis of all the evoked synaptic potentials individually within a sweep, and the analysis of the entire train-evoked synaptic response as a single entity. (2) Multitasking can be used to run a Continuous Acquisition task (saving data to a gap-free Axon Binary File), while concurrently running the Stimulation/Acquisition Sweeps task. (3) Dynamic Protocol Scripting can be used to make more complicated protocols involving nested Loops (with counters), Delays, Sweeps (with various stimulations), and Run functions (which execute a block of functions). Protocol flow can be changed while the experiment is running. WinLTP runs on National Instruments M-Series and Molecular Devices Digidata 132x boards, and is available at www.winltp.com. (C) 2007 Elsevier B.V. All rights reserved.