Effects of ouabain on respiratory rhythm generation in brainstem-spinal cord preparation from newborn rats and in decerebrate and arterially perfused in situ preparation from juvenile rats.
Effects of ouabain on respiratory rhythm generation in brainstem-spinal cord preparation from newborn rats and in decerebrate and arterially perfused in situ preparation from juvenile rats.
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哇巴因对新生大鼠脑干脊髓制备物和幼年大鼠去脑和动脉灌注原位制备物中呼吸节律产生的影响。
DOI：
10.1016/j.neuroscience.2014.12.006
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发表时间：
2015
期刊：
影响因子：
3.3
通讯作者：
Kawakami K. and Onimaru H.
中科院分区：
文献类型：
--
作者：
Tsuzawa K.;Yazawa I.;Shakuo T.;Ikeda K.;Kawakami K. and Onimaru H.
Na/K-ATP酶在呼吸节律产生中的意义尚不清楚。我们研究了Na/K-ATP酶阻断剂哇巴因对呼吸节律的影响。实验用0 ~ 3日龄Wistar大鼠脑干-脊髓标本和幼年大鼠（出生后11-13日龄）去脑动脉灌注原位标本进行。在26-27 °C下，以3.0 ml/min的速率用95% O2和5% CO2（pH 7.4）平衡的人工脑脊液灌注新生大鼠制备物。从第四颈前根（C4）监测吸气活动。哇巴因的应用（15-20分钟）导致C4吸气活动的爆发率呈剂量依赖性增加。洗脱后，每种条件下的突释率进一步增加，达到准最大值（例如，洗脱30 min时，1 μM为对照品的183%，10 μM为对照品的253%，20 μM为对照品的303%）。延髓头端腹外侧区的吸气或吸气前神经元被去极化。我们在幼年大鼠的原位灌注制备中获得了类似的结果（即膈爆发率增加）。Na/K-ATP酶α亚基基因在新生大鼠面旁呼吸组（parasfacial respiratory group，pFRG）区域表达，提示pFRG内的神经元和/或胶质细胞是哇巴因作用的靶细胞之一。结论：Na/K-ATP酶活性可能是呼吸节律调节的重要因素。
The significance of Na/K-ATPase on respiratory rhythm generation is not well understood. We investigated the effects of the Na/K-ATPase blocker, ouabain, on respiratory rhythm. Experiments were performed with brainstem-spinal cord preparation from 0 to 3-day-old Wistar rats and with decerebrate and arterially perfusedin situpreparation from juvenile rats (postnatal day 11–13). Newborn rat preparations were superfused at a rate of 3.0 ml/min with artificial cerebrospinal fluid, equilibrated with 95% O2and 5% CO2, pH 7.4, at 26–27 °C. Inspiratory activity was monitored from the fourth cervical ventral root (C4). Application of ouabain (15–20 min) resulted in a dose-dependent increase in the burst rate of C4 inspiratory activity. After washout, the burst rate further increased to reach quasi-maximum values under each condition (e.g. 183% of control in 1 μM, 253% in 10 μM, and 303% in 20 μM at 30 min washout). Inspiratory or pre-inspiratory neurons in the rostral ventrolateral medulla were depolarized. We obtained similar results (i.e. increased phrenic burst rate) in anin situperfused preparation of juvenile rats. Genes encoding the Na/K-ATPase α subunit were expressed in the region of the parafacial respiratory group (pFRG) in neonatal rats, suggesting that cells (neurons and/or glias) in the pFRG were one of the targets of ouabain. We concluded that Na/K-ATPase activity could be an important factor in respiratory rhythm modulation.