Fullerene-C60/liposome complex: Defensive effects against UVA-induced damages in skin structure, nucleus and collagen type I/IV fibrils, and the permeability into human skin tissue
Fullerene-C60/liposome complex: Defensive effects against UVA-induced damages in skin structure, nucleus and collagen type I/IV fibrils, and the permeability into human skin tissue
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DOI：
10.1016/j.jphotobiol.2009.11.010
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发表时间：
2010-01-21
影响因子：
5.4
通讯作者：
Miwa, Nobuhiko
中科院分区：
文献类型：
--
作者：
Kato, Shinya;Aoshima, Hisae;Miwa, Nobuhiko
We previously reported biological safety of fullerene-C60 (C60) incorporated in liposome consisting of hydrogenated lecithin and glycine soja sterol. as Liposome-Fullerene (0.5% aqueous phase, a particle size, 76 nm; Lpsm-Flln), and its cytoprotective activity against UVA. In the present study, LPsm-Flln was administered on the Surface of three-dimensional human skin tissue model. rinsed out before UVA-irradiation at 4J/cm(2), and thereafter added again, followed by 19-cycle-repetition for 4 days (sum 76 J/cm(2)). UVA-caused corneum scaling and disruption of epidermis layer were detected by scanning electron microscopy. Breakdown of collagen type I/IV. DNA strand cleavage and pycnosis/karyorhexis were observed in vertical cross-sections of UVA-irradiated skin models Visualized with fluorescent immunostain or Hoechst 33342 stain These skin damages were scarcely repressed by liposome alone, but appreciably repressed by Lpsm-Flln of 250 ppm, containing 0.75 ppm of C60-equivalent to a 1/3300-weight amount vs the whole liposome. Upon administration with Lpsm-Flln [16 7 mu M (12 ppm). C60-equivalent] on human abdomen skin biopsies mounted in Franz diffusion cells. C60 permeated after 24 h into the epidermis at 1.86 nmol/g tissue (1 34 ppm), corresponding to 0.3% of the applied amount and a 9.0-fold dilution rate. but C60 was not detected in the dermis by HPLC, suggesting no necessity for considering a toxicity of C60 due to systemic circulation via dermal veins Thus Lpsm-Flln has a potential to be safely utilized as a cosmetic anti-oxidative ingredient for UVA-protection (C) 2009 Elsevier B.V. All rights reserved
