A Simple Identification Method of Saliva by Detecting Streptococcus salivarius Using Loop‐Mediated Isothermal Amplification
A Simple Identification Method of Saliva by Detecting Streptococcus salivarius Using Loop‐Mediated Isothermal Amplification
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DOI：
10.1111/j.1556-4029.2010.01579.x
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发表时间：
2011-01
影响因子：
1.6
通讯作者：
H. Nakanishi;T. Ohmori;M. Hara;A. Takada;H. Shojo;Noboru Adachi;Kazuyuki Saito
中科院分区：
文献类型：
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作者：
H. Nakanishi;T. Ohmori;M. Hara;A. Takada;H. Shojo;Noboru Adachi;Kazuyuki Saito
翻译后摘要：我们以前曾报道，唾液链球菌的检测是可行的，证明在法医样本中的唾液的存在。本文介绍了一种简便、快速的检测沙门氏菌的方法。开发了使用环介导等温扩增（LAMP）检测法医样品中唾液腺病毒的方法。LAMP引物组采用S.唾液-葡萄糖基转移酶K的特异性序列。为简化操作，样品制备仅采用煮沸和变溶菌素处理，整个分析过程在2.5 h内完成。反应终止后，将截止值设定为0.1吸光度单位，在660 nm处测量。S.在所有唾液样本中都发现了唾液腺，但在其他体液或皮肤表面没有发现。利用这种方法，S.在各种模拟法医样品中成功检测到唾液腺。因此，我们建议，这种方法是有用的鉴定唾液在法医实践中。
Abstract: We previously reported that detection of Streptococcus salivarius is feasible for proving the presence of saliva in a forensic sample. Here, a simple and rapid method for the detection of S. salivarius in forensic samples was developed that uses loop‐mediated isothermal amplification (LAMP). The LAMP primer set was designed using S. salivarius‐specific sequences of glucosyltransferase K. To simplify the procedure, the sample was prepared by boiling and mutanolysin treatment only, and the entire analytical process was completed within 2.5 h. The cut‐off value was set at 0.1 absorbance units, measured at 660 nm, upon termination of the reaction. S. salivarius was identified in all saliva samples, but was not detected in other body fluids or on the skin surface. Using this method, S. salivarius was successfully detected in various mock forensic samples. We therefore suggest that this approach is useful for the identification of saliva in forensic practice.