Methods for growing and titrating African swine fever virus: field and laboratory samples.
Methods for growing and titrating African swine fever virus: field and laboratory samples.
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DOI：
10.1002/0471143030.cb2614s53
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发表时间：
2011-12-01
影响因子：
--
通讯作者：
de Leon, Patricia
中科院分区：
文献类型：
--
作者：
Carrascosa, Angel L;Bustos, M Jose;de Leon, Patricia
非洲猪瘟病毒（ASFV）分离株主要从田间获得，但也在实验室中进行工程改造，是诊断，滴定或病毒感染研究的关键步骤。本单元描述了一套在细胞培养物中生产和滴定任何ASFV毒株的方法和方案。这些程序包括（1）制备病毒敏感靶细胞的基本技术;（2）病毒储备液的生长、浓缩和纯化策略;以及（3）用于测定病毒滴度的半定量（终末稀释）和定量（空斑）试验，以及使用不同的ASFV敏感细胞作为病毒生产和滴定的靶标。
Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration.