Membrane permeability of isolated lung cells to nonelectrolytes at different temperatures.
Membrane permeability of isolated lung cells to nonelectrolytes at different temperatures.
复制标题
不同温度下分离肺细胞对非电解质的膜渗透性。
DOI：
10.1152/ajpcell.1982.243.5.c285
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
F. Chinard
中科院分区：
文献类型：
--
作者：
R. A. Garrick;F. Chinard
用串并路模型测定了兔肺上皮细胞、内皮细胞和犬肺巨噬细胞在10~37℃温度范围内对氚水、n-[14C]醇和[14C]安替比林的膜通透性系数(P0)。在混合细胞制剂中，对水的扩散渗透率(37℃时为755×10~(-5)cm·s~(-1))和对温度变化的响应(表观活化能，Ea，10kcal.mol~(-1))均大于巨噬细胞的相应数值(分别为110×10~(-5)cm·s~(-1)和4.8kcal.mol~(-1))。在两种细胞制剂中，小分子醇(C_1-C_3)的渗透系数相似，并且比水的渗透系数高得多。安替比林和大剂量醇在混合肺细胞中的通透性系数和温度依赖性与巨噬细胞中的值不同。我们的结果与红细胞和Novikoff肝癌细胞的结果相比较，表明每种细胞制剂的透水性不同，而细胞制剂中更亲油的溶质在渗透方面是相似的。这些差异在比较在分离的细胞系统和完整的组织和器官中获得的结果时可能很重要。
Membrane permeability coefficients (P0) of rabbit lung cells consisting primarily of alveolar epithelial and endothelial cells and of alveolar macrophages from dog lungs were determined for tritiated water, n-[14C]alcohols, and [14C]antipyrine over the temperature range 10 to 37 degrees C with the series-parallel pathway model. In the mixed cell preparation both the diffusional permeability to water (755 X 10(-5) cm.s-1 at 37 degrees C) and the response to temperature change (apparent activation energy, Ea, 10 kcal.mol-1) are greater than the corresponding values in the macrophages (110 X 10(-5) cm.s-1 and 4.8 kcal.mol-1, respectively). The permeability coefficients for the small alcohols (C1-C3) are similar and considerably higher than for water in both cellular preparations. The values of the permeability coefficients and the temperature dependence for antipyrine and the larger alcohols in the mixed lung cells differ from the values obtained in the macrophages. Comparison of our results with those obtained in erythrocytes and Novikoff hepatoma cells demonstrates the differences in water permeability in each cell preparation and the similarity in permeation for the more lipophilic solutes in the cell preparations. These differences may be important in the comparison of results obtained in isolated cellular systems and in intact tissues and organs.