Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species
Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species
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蓖麻硬蜱的抗原谱：发育阶段、摄食时间和不同宿主物种反应的影响
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
P. Nuttall
中科院分区：
文献类型：
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作者：
C. Lawrie;P. Nuttall
寄主物种对体外寄生虫侵袭的反应所识别的抗原已被广泛报道。虽然已经描述了不同宿主物种免疫反应的差异，但这些研究中只有很少的几项比较了不同宿主物种对侵染反应识别的抗原范围。我们用免疫印迹分析研究了不同寄主物种对反复感染蓖麻硬蜱的抗原性反应。用反复感染成虫、若虫或幼虫的兔血清，比较了幼虫和若虫全血清液和相应的唾液腺提取液(SGE)的抗原谱。SGE样本也用成虫、若虫或幼虫出没的仓鼠的血清进行了分析。用反复感染幼虫的BALB/C小鼠、黄颈姬鼠(Apodemus Huang Icollis)和银纹夜蛾(Clerionomys Tarreolus)的血清比较了SGE和幼虫匀浆的抗原性。我们还使用兔血清，通过比较雌性成虫的中肠提取液、未喂食的硬蜱的SGE和整个6天取食期的硬蜱与雌、雄成虫、若虫和幼虫的完整硬质匀浆，研究了硬蜱抗原的不同来源。被寄主种类识别的抗原性壁虱分子模式因取食期、发育阶段和寄生寄主种类的不同而不同。
Antigens recognized by host species in response to ectoparasite infestation have been widely reported. Although differences in the immune responses of different host species have been described, only a very few of these studies compare the range of antigens recognized by different host species in response to infestation. We used Western blot analysis to investigate antigenic responses of different host species that were repeatedly infested with Ixodes ricinus ticks. Antigenic profiles of larval and nymphal whole tick homogenates were compared with the respective salivary gland extract (SGE) samples using sera from rabbits repeatedly infested with either adults, nymphs or larvae. SGE samples were also analysed using sera from hamsters infested with adults, nymphs or larvae. Sera from BALB/C mice, Apodemus flavicollis (yellow‐necked mouse) or Clethrionomys glareolus (bank vole) repeatedly infested with larvae were used to compare the antigenic profiles of SGE and larval homogenate samples. We also investigated different sources of tick antigens, using rabbit sera, by comparing midgut extracts from female adult ticks and SGE from unfed ticks and from ticks throughout the 6‐day feeding period with whole tick homogenates of female and male adults, nymphs and larvae. The pattern of antigenic tick‐molecules recognized by infested host species varies with the period of feeding, developmental stage and the particular host species parasitized.
皮肤嗜碱性粒细胞相关的蜱抗性的免疫血清转移：7SIgG1 抗体的介导。
DOI：
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发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Brown,SJ;Graziano,FM;Askenase,PW
通讯作者：
Askenase,PW