Cloning and characterization of a novel UDP-GaINAc:polypeptide N-acetylgalactosaminyltransferase, pp-GaINAc-T14
Cloning and characterization of a novel UDP-GaINAc:polypeptide N-acetylgalactosaminyltransferase, pp-GaINAc-T14
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DOI：
10.1016/s0006-291x(02)02908-x
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发表时间：
2003-01-17
影响因子：
3.1
通讯作者：
Narimatsu, H
中科院分区：
文献类型：
--
作者：
Wang, H;Tachibana, K;Narimatsu, H
克隆了人UDP-N-乙酰-D-半乳糖胺：多肽N-乙酰半乳糖胺转移酶（pp-GalNAc-T）基因家族的新成员，并命名为pp-GalNAc-T14。该II型膜蛋白包含pp-GalNAc-T家族蛋白中保守的所有基序，并在系统发育树上与pp-GalNAc-T2形成一个亚家族。定量真实的时间PCR分析揭示pp-GalNAc-T14转录物在肾脏中的显著高表达，尽管转录物在所检查的所有组织中普遍表达。此外，重组pp-GalNAc-T14将GalNAc转移至一组粘蛋白衍生的肽底物，例如Muc 2、Muc 5AC、Muc 7和Muc 13（-58）。结果表明pp-GalNAc-T14是pp-GalNAc-T家族的一个新成员，并可能参与了肾脏的O-糖基化。(C)2002 Elsevier Science（美国）。All rights reserved.
A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned and designated pp-GalNAc-T14. This type II membrane protein contains all motif's that are conserved in the pp-GalNAc-T family proteins and forms a subfamily with pp-GalNAc-T2 on the phylogenetic tree. Quantitative real time PCR analysis revealed significantly high expression of the pp-GalNAc-T14 transcript in kidney, although the transcripts were ubiquitously expressed in all tissues examined. Furthermore, the recombinant pp-GalNAc-T14 transferred GalNAc to a panel of mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). Our results provide evidence that pp-GalNAc-T14 is a new member of the pp-GalNAc-T family and suggest that pp-GalNAc-T14 may be involved in the O-glycosylation in kidney. (C) 2002 Elsevier Science (USA). All rights reserved.