CENH3 distribution and differential chromatin modifications during pollen development in rye (Secale cereale L.)
CENH3 distribution and differential chromatin modifications during pollen development in rye (Secale cereale L.)
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DOI：
10.1007/s10577-011-9207-6
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发表时间：
2011-05-01
影响因子：
2.6
通讯作者：
Hause, Gerd
中科院分区：
文献类型：
--
作者：
Houben, Andreas;Kumke, Katrin;Hause, Gerd
被子植物的小配子发生产生两个结构和功能不同的细胞，一个是生殖细胞，随后形成精子细胞，另一个是营养细胞。对黑麦花粉第一次和第二次有丝分裂后两种核的染色质特性进行了分析。生殖核的浓缩染色质的显著特征是组蛋白H3K4/K9二甲基化和H3K9乙酰化水平的提高。较少浓缩的营养核呈RNA聚合酶II阳性。H3K27的三甲基化不参与生殖核中基因的转录下调，因为H3K27me3只在营养核中检测到。DNA甲基化的总体水平在两种花粉核之间没有差异。在黑麦中，与拟南芥不同（Ingouff等）。现代生物杂志（17:10 -10）；Schoft等人。EMBO Rep 10:1015-1021 2009)，着丝粒组蛋白H3不排除在营养核的染色质中，着丝粒和亚端粒区域的凝聚程度在生殖核和营养核之间没有差异。黑麦和拟南芥的数据差异表明，花粉粒成熟核的染色质组织在被子植物中并不普遍。
Microgametogenesis in angiosperms results in two structurally and functionally different cells, one generative cell, which subsequently forms the sperm cells, and the vegetative cell. We analysed the chromatin properties of both types of nuclei after first and second pollen mitosis in rye (Secale cereale). The condensed chromatin of generative nuclei is ear-marked by an enhanced level of histone H3K4/K9 dimethylation and H3K9 acetylation. The less condensed vegetative nuclei are RNA polymerase II positive. Trimethylation of H3K27 is not involved in transcriptional downregulation of genes located in generative nuclei as H3K27me3 was exclusively detected in the vegetative nuclei. The global level of DNA methylation does not differ between both types of pollen nuclei. In rye, unlike in Arabidopsis thaliana (Ingouff et al. Curr Biol 17:1032-1037 2007; Schoft et al. EMBO Rep 10:1015-1021 2009), centromeric histone H3 is not excluded from the chromatin of the vegetative nucleus and the condensation degree of centromeric and subtelomeric regions did not differ between the generative and vegetative nuclei. Differences between rye and A. thaliana data suggest that the chromatin organization in mature nuclei of pollen grains is not universal across angiosperms.