GASTRIC-ACID SECRETION AND LESION FORMATION IN RATS UNDER WATER-IMMERSION STRESS
GASTRIC-ACID SECRETION AND LESION FORMATION IN RATS UNDER WATER-IMMERSION STRESS
复制标题
DOI：
10.1007/bf01300703
复制
发表时间：
1986-02-01
影响因子：
3.1
通讯作者：
TAKEUCHI, K
中科院分区：
文献类型：
--
作者：
HAYASE, M;TAKEUCHI, K
为探讨胃酸在应激性胃损伤发病机制中的作用，本实验研究了幽门结扎和胃腔灌流大鼠在单纯束缚(R)和束缚加水浸泡(WI)条件下胃酸分泌的变化。采用氨基比林清除法测定急性肠瘘大鼠胃粘膜血流量(GMBF)。幽门结扎大鼠在3.5小时或7小时的应激状态下，胃酸分泌明显减少。在肠腔灌流或急性肠瘘大鼠中，应激7小时也有类似的下降；而在WI组，应激期间3-4小时的胃酸分泌显著增加，但未超过应激水平。仅在WI组，GMBF表现出与酸性分泌活性相似的增加，这种增加可被阿托品(1 mg/kg)或西咪替丁(60 mg/kg)显著抑制。两组患者在3.5小时时均出现胃部病变，仅在WI组在7小时时出现广泛严重的胃部病变。西咪替丁在3.5小时未能影响损伤的形成，但在7小时显著抑制了随后的损伤生长，而阿托品或幽门结扎几乎完全阻止了3.5小时或7小时的WI应激诱导的损伤。这些结果表明，大鼠暴露于应激(R或WI)后，胃酸分泌活性普遍下降，但在WI应激过程中，胃酸分泌恢复到正常水平，这可能在应激性胃损伤的加重过程中起重要作用。
In attempts to investigate the roles of acid in the pathogenesis of stress-induced gastric lesions, gastric acid secretion was studied in pylorus-ligated and lumen-perfused rats under restraint alone (R) or restraint with additional water immersion (WI). Gastric mucosal blood flow (GMBF) was measured with the aminopyrine clearance method in acute fistula rats. Acid secretion in pylorus-ligated rats significantly decreased under R or WI of either 3.5 or 7 hr stress. In the lumen-perfused or acute-fistula rats, exposure of rats to stress for 7 hr produced a similar decrease; however, in the WI group, there was a significant increase of acid secretion for 3-4 hr during stress, but not exceeding the prestress level. Only in the WI group did GMBF exhibit similar increases to those of acid secretory activity, and these increases were significantly inhibited by intraperitoneal administration of atropine (1 mg/kg) or cimetidine (60 mg/kg). Gastric lesions developed in both groups at 3.5 hr and became extensively severe at 7 hr only in the WI group. Cimetidine failed to influence the formation of lesions at 3.5 hr but significantly inhibited the later outgrowth of lesions at 7 hr, while atropine or pylorus ligation all but completely prevented lesions induced by either 3.5- or 7-hr WI stress. These results indicate that exposure of rats to stress (R or WI) generally decreased acid secretory activity, but there was a rise in acid secretion toward normal levels during WI stress, which may play an important role in the aggravating process of stress-induced gastric lesions.