2 CELL-LINES FROM BONE-MARROW THAT DIFFER IN TERMS OF COLLAGEN-SYNTHESIS, OSTEOGENIC CHARACTERISTICS, AND MATRIX MINERALIZATION
2 CELL-LINES FROM BONE-MARROW THAT DIFFER IN TERMS OF COLLAGEN-SYNTHESIS, OSTEOGENIC CHARACTERISTICS, AND MATRIX MINERALIZATION
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DOI：
10.2106/00004623-199301000-00012
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发表时间：
1993-01-01
影响因子：
5.3
通讯作者：
BALIAN, G
中科院分区：
文献类型：
--
作者：
DIDUCH, DR;COE, MR;BALIAN, G
从小鼠骨髓细胞培养中分离出两个克隆细胞系。其中D1系表现出成骨特性，并合成i型胶原（alpha1）2alpha2。第二个细胞系D2不成骨，并产生胶原同源三聚体（α 1）3。而D1细胞培养的细胞外基质含有条纹胶原原纤维，可能由i型胶原组成，而产生同源三聚体的D2细胞没有显示出条纹胶原原纤维。相反，它们有细丝，没有明显的条纹。抗坏血酸钠在D1和D2细胞的转录水平上刺激胶原合成。D1体外成骨的特点包括高水平的碱性磷酸酶，甲状旁腺激素治疗后环磷酸腺苷升高，骨钙素mRNA表达。与D2细胞不同，D1细胞在体外产生矿化基质。原位杂交显示，D1细胞培养中的矿化发生在细胞密度增加的结节中，其中也含有胶原mRNA浓度最高的细胞。将D1细胞植入体内扩散室，形成成骨和成脂混合组织。这表明D1细胞系来源于多电位的早期骨髓基质前体细胞。临床意义：D1细胞的成骨能力证实骨髓细胞具有成骨作用。来自骨髓的细胞似乎参与了骨折愈合和骨长入假体植入物。D1细胞系可能是一个合适的系统，在体外和体内研究与骨形成有关的细胞和生化事件，以及生长因子、激素和药物的影响。
Two cloned cell lines were isolated from cultures of mouse bone-marrow cells. One of the lines, D1, exhibited osteogenic properties and synthesized type-I collagen (alpha1)2alpha2. The second cell line, D2, was not osteogenic and produced a collagen homotrimer (alpha1)3. Whereas the extracellular matrix of the D1 cell cultures contained striated collagen fibrils, presumably composed of type-I collagen, the homotrimer-producing D2 cells did not demonstrate striated collagen fibrils. Instead, they had thin filaments without detectable striations. Sodium ascorbate stimulated collagen synthesis at the transcriptional level in both the D1 and the D2 cells. The bone-producing characteristics of D1 in vitro included high levels of alkaline phosphatase, increased cyclic adenosine monophosphate on treatment with parathyroid hormone, and expression of osteocalcin mRNA. The D1 cells, unlike the D2 cells, produced a mineralized matrix in vitro. Mineralization in the cultures of the D1 cells occurred in nodules of increased cell density, which also contained the cells with the highest concentrations of collagen mRNA, as shown by in situ hybridization. When the D1 cells were implanted in a diffusion chamber in vivo, a mixture of both osteogenic and adipogenic tissues was formed.This indicates that the D1 cell line is derived from an early marrow stromal precursor that is multipotential.CLINICAL RELEVANCE: The bone-forming capacity of D1 cells confirms that bone marrow cells are osteogenic. Cells from the bone marrow appear to participate in fracture-healing and ingrowth of bone into prosthetic implants. The D1 cell line may be a suitable system in which to study, both in vitro and in vivo, the cellular and biochemical events associated with formation of bone as well as the effects of growth factors, hormones, and drugs.