Genome Sequence Analysis of CsRV1: A Pathogenic Reovirus that Infects the Blue Crab Callinectes sapidus Across Its Trans-Hemispheric Range.
Genome Sequence Analysis of CsRV1: A Pathogenic Reovirus that Infects the Blue Crab Callinectes sapidus Across Its Trans-Hemispheric Range.
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DOI：
10.3389/fmicb.2016.00126
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发表时间：
2016
影响因子：
5.2
通讯作者：
Schott EJ
中科院分区：
文献类型：
--
作者：
Flowers EM;Bachvaroff TR;Warg JV;Neill JD;Killian ML;Vinagre AS;Brown S;Almeida AS;Schott EJ
蓝蟹Callinectes sapidus Rathbun，1896是西大西洋沿岸生态系统中重要的商业营养链，在北美和南美都受到C. sapidus呼肠孤病毒1（CsRV 1），一种双链RNA病毒。采用离子激流技术对CsRV 1北美株的12个基因组片段进行测序。基于它们与其他呼肠孤病毒基因组中编码的蛋白质的相似性，可以为基因组中编码的13种蛋白质中的3种分配推定的功能。CsRV 1依赖于RNA的RNA聚合酶（RdRP）序列与其他感染螃蟹的呼肠孤病毒的基因组的比较显示，它与锯缘青蟹中发现的泥蟹呼肠孤病毒和中华绒螯蟹中发现的WX-2012相似，并支持存在一个独特的“Crabreo”属的想法，不同于Seadornavirus和Cardoreovirus，这两个最接近的属在呼肠孤病毒科。CsRV 1与Macropipus defurator的P病毒的唯一可用序列之间的98%核苷酸序列同一性的区域表明，这两种病毒可能密切相关。从15个感染的中华绒螯蟹中扩增了CsRV 1 RdRP基因的860个核苷酸的区域，并进行了测序。sapidus。预测的蛋白质序列的成对分析表明，CsRV 1株在巴西可以区分从那些在北美的基础上保守的残基在这个基因。CsRV 1基因组的测序、注释和初步的群体指标将促进不同学科的进一步研究，包括呼肠孤病毒蛋白的结构-功能关系、对呼肠孤病毒科进化的调查以及对C.分布在北方和南半球。
The blue crab, Callinectes sapidus Rathbun, 1896, which is a commercially important trophic link in coastal ecosystems of the western Atlantic, is infected in both North and South America by C. sapidus Reovirus 1 (CsRV1), a double stranded RNA virus. The 12 genome segments of a North American strain of CsRV1 were sequenced using Ion Torrent technology. Putative functions could be assigned for 3 of the 13 proteins encoded in the genome, based on their similarity to proteins encoded in other reovirus genomes. Comparison of the CsRV1 RNA-dependent RNA polymerase (RdRP) sequence to genomes of other crab-infecting reoviruses shows that it is similar to the mud crab reovirus found in Scylla serrata and WX-2012 in Eriocheir sinensis, Chinese mitten crab, and supports the idea that there is a distinct “Crabreo” genus, different from Seadornavirus and Cardoreovirus, the two closest genera in the Reoviridae. A region of 98% nucleotide sequence identity between CsRV1 and the only available sequence of the P virus of Macropipus depurator suggests that these two viruses may be closely related. An 860 nucleotide region of the CsRV1 RdRP gene was amplified and sequenced from 15 infected crabs collected from across the geographic range of C. sapidus. Pairwise analysis of predicted protein sequences shows that CsRV1 strains in Brazil can be distinguished from those in North America based on conserved residues in this gene. The sequencing, annotation, and preliminary population metrics of the genome of CsRV1 should facilitate additional studies in diverse disciplines, including structure-function relationships of reovirus proteins, investigations into the evolution of the Reoviridae, and biogeographic research on the connectivity of C. sapidus populations across the Northern and Southern hemispheres.