Electrochemical Behavior of Paraquat on a Highly Ordered Biosensor Based on an Unmodified DNA-3D Gold Nanoparticle Composite and Its Application
Electrochemical Behavior of Paraquat on a Highly Ordered Biosensor Based on an Unmodified DNA-3D Gold Nanoparticle Composite and Its Application
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百草枯在基于未修饰 DNA-3D 金纳米粒子复合材料的高度有序生物传感器上的电化学行为及其应用
DOI：
10.1016/j.electacta.2014.11.191
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发表时间：
2015-01
影响因子：
6.6
通讯作者：
Weijun Kang
中科院分区：
文献类型：
--
作者：
Kaoqi Lian;Li Ma;Hongmei Shi;Weijun Kang
DNA通常利用DNA分子一端的巯基或氨基吸附金纳米颗粒。然而，在这项研究中，我们报告了一种高度有序的生物传感器，该生物传感器使用未修饰的DNA分子与连续的腺嘌呤和三维金纳米颗粒（3D GNP）构建。未修饰的DNA-3D GNP复合材料在金电极上制备，并通过使用扫描电子显微镜（SEM），原子力显微镜（AFM）和电化学方法进行表征。利用电化学石英晶体微天平（EQCM），未修饰的DNA和GNP结合的机制进行了研究。该修饰电极对百草枯具有超灵敏的响应。用循环伏安法（CV）和微分脉冲伏安法（DPV）研究了还原峰电流与浓度的线性关系。DPV的线性关系为7.0 × 10− 9 M至1.5 × 10− 6 M，检测限为2.0 × 10− 10 M。同时，还探讨了百草枯在该修饰电极上的氧化还原机理。该方法用于人血清、人尿液和天然样品的检测，获得了满意的结果。
DNA usually adsorbs gold nanoparticle by virtue of mercapto or amino group at one end of a DNA molecule. However, in this study, we report a highly ordered biosensor constructed using unmodified DNA molecules with consecutive adenines and three-dimensional gold nanoparticles (3D GNPs). The unmodified DNA-3D GNP composite was fabricated on gold electrodes and characterized through the use of scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical methods. Using an electrochemical quartz crystal microbalance (EQCM), the mechanism by which the unmodified DNA and GNPs combined was also studied. The modified electrode exhibited an ultrasensitive response to paraquat. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the linear relationships between the concentrations and the reduction peak currents. The linear relationship for DPV is 7.0 × 10−9M to 1.5 × 10−6M with a detection limit of 2.0 × 10−10M. The redox mechanism of paraquat on this modified electrode was also elucidated. The feasibility of the proposed assay for use in human serum, human urine, and natural samples was investigated, and satisfactory results were obtained.
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