Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs.
Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs.
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DOI：
10.1186/s12917-016-0725-5
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发表时间：
2016-06-10
影响因子：
2.6
通讯作者：
Zimmerman J
中科院分区：
文献类型：
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作者：
Bjustrom-Kraft J;Woodard K;Giménez-Lirola L;Rotolo M;Wang C;Sun Y;Lasley P;Zhang J;Baum D;Gauger P;Main R;Zimmerman J
来自两个生产地点的纵向样本被用来(1)描述PEDV在单个直肠拭子、PEN粪便样本和PEDV口腔液(OF)中的脱落模式；(2)通过对猪血清和PEDV口腔液样本的ELISA(IgA、IgG)检测来描述PEDV抗体的动力学；以及(3)建立PEDV WV ELISA(IgA、IgG)的临界值和性能评估。第一个部位PEDV阳性，第二个部位PEDV阴性。在第一个地点，在人群接触PEDV之前和之后都收集了围栏样本(粪便和口腔液)和猪样本(直肠拭子和血清)。在第二点，PEDV抗体和核酸均为阴性。在第一点，所有样本在暴露后6天(DPE)用RRT-PCR方法检测到PEDV。69例DPE患者的直肠拭子和口腔液中均检出RRT-PCR阳性。用13DPE检测口腔液和血清中的免疫球蛋白Ig G和Ig A。对PEDV血清免疫球蛋白WV-EL ISA数据的分析表明，样本对阳性(S/P)的临界值 ≥ 为0.80时，诊断的敏感性为0.87(95%CI：0.82，0.91)，特异性为0.99(95%CI：0.98，1.00)。在监测期间，血清免疫球蛋白结果缓慢下降，在111DPE的末次采样时，60%的血清样本呈阳性(S/P ≥ 0.80)。以S/P ≥ 0.80为界值，诊断的敏感性为1.00(95%CI：0.92，1.00)，特异性为1.00(95%CI：0.99，1.00)。口服液免疫球蛋白A反应在96DPE时升高，在最后一次采样时开始下降。这项研究表明，基于口服液的检测可以提供一种简单且对动物友好的方法来采集样本，用于猪群体中PEDV的核酸和/或抗体监测。
Longitudinal samples from two production sites were used to (1) describe the pattern of PEDV shedding (rRT-PCR) in individual rectal swabs, pen fecal samples, and pen oral fluids (OF); (2) describe the kinetics of PEDV antibody by ELISA (IgA, IgG) testing of pig serum and pen oral fluid samples; and (3) establish cutoffs and performance estimates for PEDV WV ELISAs (IgA, IgG). Site One was PEDV positive; Site Two was PEDV negative. On Site One, pen samples (feces and oral fluids) and pig samples (rectal swabs and sera) were collected both before and after the population was exposed to PEDV. On Site Two, pen oral fluid samples and individual pig serum samples were negative for both PEDV antibody and nucleic acid. On Site One, PEDV was detected by rRT-PCR at 6 days post exposure (DPE) in all sample types. The last rRT-PCR positives were detected in rectal swabs and oral fluids on 69 DPE. IgG and IgA were detected in oral fluids and serum samples by 13 DPE. Analysis of the PEDV serum IgG WV ELISA data showed that a sample-to-positive (S/P) cutoff of ≥ 0.80 provided a diagnostic sensitivity of 0.87 (95 % CI: 0.82, 0.91) and specificity of 0.99 (95 % CI: 0.98, 1.00). Serum IgG results declined slowly over the monitoring period, with 60 % of serum samples positive (S/P ≥ 0.80) at the final sampling on 111 DPE. Analysis of the PEDV oral fluid IgA WV ELISA found that a cutoff of S/P ≥ 0.80 provided a diagnostic sensitivity of 1.00 (95 % CI: 0.92, 1.00) and a diagnostic specificity of 1.00 (95 % CI: 0.99, 1.00). The oral fluid IgA response increased through 96 DPE and began to decline at the last sampling on 111 DPE. This study showed that oral fluid-based testing could provide an easy and “animal-friendly” approach to sample collection for nucleic acid and/or antibody-based surveillance of PEDV in swine populations.