Cyclin D1 amplification correlates with early recurrence of squamous cell carcinoma of the tongue
Cyclin D1 amplification correlates with early recurrence of squamous cell carcinoma of the tongue
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DOI：
10.1016/s0304-3835(01)00651-6
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发表时间：
2001-10-30
期刊：
影响因子：
9.7
通讯作者：
Tashiro, M
中科院分区：
文献类型：
--
作者：
Fujii, M;Ishiguro, R;Tashiro, M
采用石蜡包埋标本，用荧光原位杂交(FISH)技术研究了23例舌癌组织中CCND1基因的扩增情况。所有患者均行原发灶全切除加或不加颈淋巴清扫术。CCND1扩增阳性率为56.5%(1.3/23)。CCND1扩增与组织学分级、T分类、N分类和分期无显著相关性。CCND1DNA扩增阳性患者的5年无瘤生存率为23.1%，阴性患者为80.0%，差异有统计学意义(P=0.0070)。以11号染色体着丝粒和11q13号染色体着丝粒为探针，对9例患者进行双色FISH检查。在5例CCND1DNA扩增阳性的患者中，11q13信号数目多于染色体I I着丝粒数目的细胞数显著高于CCND1DNA扩增阴性患者(P=0.013)。这表明在CCND1扩增阳性的患者中，11q13的扩增比I I染色体的异常更常见。从这些结果来看，CCND1的扩增是预测舌癌侵袭性的关键因素。此外，事实证明，FISH是一种有用的评估方法。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
Amplification of CCND1 was studied in 23 tongue carcinoma patients by fluorescence in situ hybridization (FISH) using a paraffin embedded specimen. All the patients received complete resection of the primary site with or without neck dissection. CCND1 amplification was positive in 1.3 (56.5%) out of 23 cases. Correlations between CCND1 amplification and histological grading, T category, N category, and Stages were not significant. The 5-year disease-free survival rate, which was 23.1% for CCND1 amplification positive patients and 80.0% for negative patients, was significantly better for the CCND1 amplification negative patients (P = 0.0070). Nine patients were examined by dual-color FISH with the probe for centromere of chromosome 11 and 11q13. In five patients, who had positive amplification for CCND1, cell numbers with a larger number of signals for 11q13 than the centromere of chromosome I I were significantly higher than those of CCND1 amplification negative patients (P = 0.013). This indicates that amplification of 11q13 occurs more frequently than aberration of chromosome I I in CCND1 amplification positive patients. From these results, the amplification of CCND1 is a key factor in predicting the aggressiveness of tongue cancer. Furthermore, FISH proved to be a useful method for such evaluation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.