Effective interactions between chaotropic agents and proteins
Effective interactions between chaotropic agents and proteins
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DOI：
10.1002/prot.20626
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发表时间：
2005-11-15
影响因子：
2.9
通讯作者：
Vendruscolo, M
中科院分区：
文献类型：
--
作者：
Salvi, G;De los Rios, P;Vendruscolo, M
离液剂是能够破坏水分子之间的氢键网络并通过削弱疏水作用降低蛋白质天然状态的稳定性的共溶质。在这项工作中，我们代表的离液剂作为一个因素，减少水分子形成的结构中的顺序量，无论是在散装和周围的疏水氨基酸的水化壳。在这个框架中，我们表明，低离液剂浓度导致蛋白质的天然状态的不稳定，高浓度诱导完全变性。我们还发现，大量有序状态的水分子的数量的减少可以给起源离液分子和蛋白质之间的有效相互作用。蛋白质2005;61：492-499. (c)2005 Wiley-Liss，Inc.
Chaotropic agents are cosolutes that can disrupt the hydrogen bonding network between water molecules and reduce the stability of the native state of proteins by weakening the hydrophobic effect. In this work, we represent the chaotropic agent as a factor that reduces the amount of order in the structures formed by water molecules, both in the bulk and the hydration shells around hydrophobic amino acids. In this framework we show that low chaotrope concentrations lead to a destabilization of the native state of proteins, and that high concentrations induce complete denaturation. We also find that the reduction of the number of bulk ordered states of water molecules can give origin to an effective interaction between chaotropic molecules and proteins. Proteins 2005;61:492-499. (c) 2005 Wiley-Liss, Inc.